Chen Shouhui, Wang Zhihua, Cui Xinyuan, Jiang Linlei, Zhi Yuee, Ding Xianting, Nie Zhihong, Zhou Pei, Cui Daxiang
Center of Food Safety Engineering and Technology Research, Shanghai, Key Laboratory of Urban Agriculture, Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
Nanoscale Res Lett. 2019 Feb 28;14(1):71. doi: 10.1186/s11671-019-2857-6.
How to fabricate scale low-cost microfluidic device for detection of biomarkers owns a great requirement. Herein, it is for the first time reported that a new microfluidic device based on bonding polydimethylsiloxane microfluidic channels onto the substrate of a screen-printed electrode with coating glass solution was fabricated for electrochemical sensing of prostate-specific antigen (PSA). Compared to traditional microfabrication processes, this method is simple, fast, low cost, and also suitable for mass production. The prepared screen-printed electrode-based microfluidic device (CASPE-MFD) was used for the detection of the PSA in human serum. The prepared CASPE-MFD had a detection limit of 0.84 pg/mL (25.8 fM) and a good linearity with PSA concentration ranging from 0.001 to 10 ng/mL, which showed a great promise platform toward the development of miniaturized, low-cost electrochemical microfluidic device for use in human health, environmental monitoring, and other applications.
如何制造用于生物标志物检测的低成本微型流体装置具有很大的需求。在此，首次报道了一种新型微型流体装置，该装置通过将聚二甲基硅氧烷微流体通道与涂覆有玻璃溶液的丝网印刷电极基板键合而成，用于前列腺特异性抗原（PSA）的电化学传感。与传统的微制造工艺相比，该方法简单、快速、成本低，并且也适用于大规模生产。所制备的基于丝网印刷电极的微型流体装置（CASPE-MFD）用于检测人血清中的PSA。所制备的CASPE-MFD的检测限为0.84 pg/mL（25.8 fM），并且与PSA浓度在0.001至10 ng/mL范围内具有良好的线性关系，这表明该平台在开发用于人类健康、环境监测和其他应用的小型化、低成本电化学微型流体装置方面具有很大的潜力。
