Blokh K O, Paskevich I F
Vopr Med Khim. 1986 Jan-Feb;32(1):134-6.
A procedure is developed for estimation of DNA microquantities in biological samples, which involved acid hydrolysis of DNA and the following microbiological estimation of its concentration. A sensitivity of the procedure was as little as 0.25 micrograms DNA per ml. At the DNA concentration 0.25-10 micrograms/ml growth of the indicator strain of bacteria exhibited the linear shape. As shown by means of the procedure developed one rat leukocyte contained 6.3 +/- 0.6 X 10(-12) g of DNA.
开发了一种用于估计生物样品中微量DNA的方法，该方法包括DNA的酸水解及其浓度的微生物学估计。该方法的灵敏度低至每毫升0.25微克DNA。在DNA浓度为0.25 - 10微克/毫升时，指示菌株的生长呈线性。通过所开发的方法表明，一只大鼠白细胞含有6.3±0.6×10⁻¹²克DNA。
