室温下，VIII因子：C的微量板显色测定法。

Room temperature, microtray chromogenic assay of factor VIII:C.

作者信息

Prowse C, Hornsey V, McKay G, Waterston Y

出版信息

Vox Sang. 1986;50(1):21-5. doi: 10.1111/j.1423-0410.1986.tb04840.x.

DOI:10.1111/j.1423-0410.1986.tb04840.x

PMID:3082074

Abstract

Commercial reagents of non-human origin were used to provide a room temperature, microtray chromogenic assay for factor VIII. The assay allows determination of factor VIII in the range 15-150% of normal plasma levels with a coefficient of variation of 3-4%. Haemophilic plasmas and factor VIII concentrates may also be assayed by varying the pre-dilution step. A good correlation was demonstrated with results obtained using an established one-stage clotting assay (r = 0.9985).

摘要

使用非人类来源的商业试剂提供了一种用于检测因子VIII的室温微量板显色测定法。该测定法可测定正常血浆水平15%至150%范围内的因子VIII，变异系数为3%至4%。通过改变预稀释步骤，也可对血友病血浆和因子VIII浓缩物进行检测。与使用既定的一期凝血测定法获得的结果具有良好的相关性（r = 0.9985）。