Chantarangkul V, Ingram G I, Thorn M B, Darby S C
Br J Haematol. 1978 Nov;40(3):471-88. doi: 10.1111/j.1365-2141.1978.tb05818.x.
An 'artificial' plasma for one-stage factor-VIII assays is made by incubating human plasma with EDTA, to destroy factor VIII, and afterwards removing the anticoagulant by dialysis. Bovine factor V is then added to a given level. In the assay, contact activation is controlled by adding contact product. It was confirmed that factor-VIII activity was destroyed and that the EDTA was freely dialysable. The fibrinogen in the treated plasma clotted normally with thrombin. Likely variation in the factor-V activity was found not to be critical. The concentration of fibrinogen and other factors was adequate. Variation between batches was small. The artificial plasma yielded assay results closely comparable to haemophilic plasma in samples with factor-VIII activities in the range 0.01--20.0 iu/ml; the mean results in the artificial system were estimated to be 0.997 x those in haemophilic plasma, with a 95% confidence interval of 0.901--1.103. Biological variability in individual assays was smaller in the artificial system than when haemophilic plasma was used. Instability at the bench was more often detected in the artificial system than in haemophilic plasma assays, but the effect was eliminated from the results by obtaining duplicated readings in a balanced order.
用于一期因子 VIII 测定的“人工”血浆是通过将人血浆与乙二胺四乙酸（EDTA）孵育以破坏因子 VIII，然后通过透析去除抗凝剂而制成的。然后将牛因子 V 添加到给定水平。在测定中，通过添加接触产物来控制接触激活。已证实因子 VIII 活性被破坏且 EDTA 可自由透析。经处理的血浆中的纤维蛋白原能正常地被凝血酶凝结。发现因子 V 活性的可能变化并不关键。纤维蛋白原和其他因子的浓度足够。批次间的差异很小。在因子 VIII 活性范围为 0.01 - 20.0 iu/ml 的样本中，人工血浆产生的测定结果与血友病血浆的结果非常相似；人工系统中的平均结果估计为血友病血浆中结果的 0.997 倍，95%置信区间为 0.901 - 1.103。与使用血友病血浆时相比，人工系统中单个测定的生物学变异性更小。与血友病血浆测定相比，在人工系统中更常检测到在实验台上的不稳定性，但通过按平衡顺序获取重复读数，该影响从结果中消除。
