Wilson C A, McNeilly A S, Yeo T
Acta Endocrinol (Copenh). 1986 Feb;111(2):235-40. doi: 10.1530/acta.0.1110235.
Pregnant mare serum gonadotrophin (PMS) normally induces a pre-ovulatory immunoreactive LH surge followed by ovulation in 27 day old rats provided they weigh more than 60 g; ovulation does not occur in the lighter rats although a pre-ovulatory LH surge can still be measured. 5HT injected into the paraventricular nucleus (PVN) can induce ovulation in these smaller rats treated with PMS and does so without appearing to stimulate extra immunoreactive LH. The LH secreted in the under 60 g PMS-treated rats is a pleiomorphic form since it cannot be detected by cytochemical bioassay, indicating abnormal biological activity, which may account for its failure to induce ovulation. After unilateral injection of 5HT into PVN, the LH was detectable by the bioassay. Injection of 5HT also stimulated the release of growth hormone and prolactin but had no significant effect on the plasma concentration of the gonadotrophins or corticosterone. Since an injection of exogenous GH converted the LH to a fully bioactive form it is suggested that GH is important in inducing the secretion of an "adult' form of LH in the peripubertal rat necessary for first ovulation and that 5HT exerts its ovulatory effect via stimulating the release of this hormone.
孕马血清促性腺激素（PMS）通常会在27日龄、体重超过60克的大鼠中诱导排卵前免疫反应性促黄体生成素（LH）激增，随后排卵；体重较轻的大鼠虽然仍可检测到排卵前LH激增，但不会排卵。向室旁核（PVN）注射5-羟色胺（5HT）可诱导这些用PMS处理的较小大鼠排卵，且这样做时似乎不会刺激额外的免疫反应性LH。在体重不足60克且经PMS处理的大鼠中分泌的LH是一种多形形式，因为通过细胞化学生物测定法无法检测到它，这表明其生物活性异常，这可能是其无法诱导排卵的原因。在向PVN单侧注射5HT后，通过生物测定法可检测到LH。注射5HT还刺激了生长激素和催乳素的释放，但对促性腺激素或皮质酮的血浆浓度没有显著影响。由于注射外源性生长激素（GH）可将LH转化为完全具有生物活性的形式，因此有人认为GH在诱导青春期前大鼠分泌“成年”形式的LH中起重要作用，这是首次排卵所必需的，并且5HT通过刺激这种激素的释放发挥其排卵作用。
