Ayadi Ahmad, Nafari Amir Hossein, Sakhaee Fatemeh, Rajabi Kimia, Ghaderi Yaser, Rahimi Jamnani Fatemeh, Vaziri Farzam, Siadat Seyed Davar, Fateh Abolfazl
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Hepatol Res. 2019 Jun;49(6):605-616. doi: 10.1111/hepr.13325. Epub 2019 Apr 7.
The presence of occult hepatitis C virus (HCV) infection (OCI) is still controversial, however, this infection cannot be ignored. Therefore, the current study aimed at assessing the OCI frequency in patients on chronic hemodialysis (CHD) and also evaluating the association between OCI incidence with clinical parameters and interferon lambda 3/4 (IFNL3/4) gene polymorphisms.
A total of 515 patients on CHD and HCV negative markers were selected. Plus- and minus-stranded HCV-RNA was tested in peripheral blood mononuclear cell samples by reverse transcription-polymerase chain reaction and then genotyped using the restriction fragment length polymorphism method.
The frequency of OCI was 11.3% in patients on CHD. Among 58 patients with OCI, 25.8%, 62.1%, and 12.1% were infected with HCV-1a, HCV-1b, and HCV-3a, respectively. The mean alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels were 31.9 ± 24.8, 32.3 ± 19.1, and 171.6 ± 88.9, respectively. None of the patients with OCI had a history of liver disease. Multivariate logistic regression analysis indicated that cholesterol, triglyceride, low-density lipoprotein, 25-hydroxyvitamin D, platelets, duration of hemodialysis, HCV subtypes, IFNL3 rs12979860 TT, IFNL3 rs8099917 GG, IFNL3 rs12980275 GG, and IFNL4 ss469415590 ΔG/ΔG genotypes were associated with OCI.
There was a moderate prevalence of OCI in Iranian patients on CHD. The current study findings indicated that this infection was associated with clinical parameters and unfavorable genotypes of IFNL3 single nucleotide polymorphisms and IFNL4 ss469415590. Further studies are required to determine the correlation between OCI incidence with clinical parameters and host genetic factors.
隐匿性丙型肝炎病毒（HCV）感染（OCI）的存在仍存在争议，然而，这种感染不容忽视。因此，本研究旨在评估慢性血液透析（CHD）患者中OCI的频率，并评估OCI发生率与临床参数以及干扰素λ3/4（IFNL3/4）基因多态性之间的关联。
共选取515例CHD患者且HCV阴性标志物检测呈阴性。通过逆转录-聚合酶链反应在外周血单个核细胞样本中检测正负链HCV-RNA，然后使用限制性片段长度多态性方法进行基因分型。
CHD患者中OCI的频率为11.3%。在58例OCI患者中，分别有25.8%、62.1%和12.1%感染了HCV-1a、HCV-1b和HCV-3a。丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶的平均水平分别为31.9±24.8、32.3±19.1和171.6±88.9。OCI患者均无肝病病史。多因素逻辑回归分析表明，胆固醇、甘油三酯、低密度脂蛋白、25-羟基维生素D、血小板、血液透析时间、HCV亚型、IFNL3 rs12979860 TT、IFNL3 rs8099917 GG、IFNL3 rs12980275 GG和IFNL4 ss469415590 ΔG/ΔG基因型与OCI相关。
伊朗CHD患者中OCI的患病率中等。本研究结果表明，这种感染与临床参数以及IFNL3单核苷酸多态性和IFNL4 ss469415590的不良基因型相关。需要进一步研究以确定OCI发生率与临床参数和宿主遗传因素之间的相关性。
