Beijnen J H, Van der Nat J M, Labadie R P, Underberg W J
Anticancer Res. 1986 Jan-Feb;6(1):39-43.
The chemical stability of members of two groups of cytostatics, mitomycins and anthracyclines, has been studied in four different cell culture media enriched with serum. Stability was determined with the use of high performance liquid chromatography. In the group of mitomycins, the 7-aminomitosanes appeared to be relatively stable during a seven days incubation period at 37 degrees C when compared to the 7-methoxy congeners. The anthracycline derivatives, 4-demethoxy-daunorubicin, doxorubicin and its 4'-analogues showed half-lives of about 10-20 hours. Doxorubicinol and daunorubicin were found to be more stable. Anthracycline degradation products could be traced with the use of thin layer chromatography. All main degradation products originate from hydrolytic reactions. No enzymatic conversions could be observed. These observations may be of importance for the correct interpretation of the effects of mitomycins or anthracyclines on cells incubated in a cell culture medium.
对两组细胞抑制剂（丝裂霉素和蒽环类药物）的化学稳定性进行了研究，研究是在四种添加血清的不同细胞培养基中进行的。稳定性通过高效液相色谱法测定。在丝裂霉素组中，与7-甲氧基同系物相比，7-氨基丝裂霉素在37℃下孵育7天期间似乎相对稳定。蒽环类衍生物4-去甲氧基柔红霉素、阿霉素及其4'-类似物的半衰期约为10 - 20小时。发现阿霉素醇和柔红霉素更稳定。蒽环类降解产物可用薄层色谱法追踪。所有主要降解产物均源于水解反应。未观察到酶促转化。这些观察结果对于正确解释丝裂霉素或蒽环类药物对细胞培养基中培养的细胞的作用可能具有重要意义。
