Weickert M J, Chambliss G H, Sugiyama H
Appl Environ Microbiol. 1986 Jan;51(1):52-6. doi: 10.1128/aem.51.1.52-56.1986.
Twelve strains of Clostridium botulinum type A and seven strains of Clostridium sporogenes were screened for plasmids by agarose gel electrophoresis of cleared lysates of cells from 5 ml of mid-log-phase culture. Nine type A strains had one or more plasmids of 4.3, 6.8, or 36 megadaltons (MDa); several strains showed a large plasmid of 61 MDa, but it was not consistently recovered. Four C. sporogenes strains had one or more plasmids of 4.3, 5.6 or 36 MDa. Isolates obtained from cultures of plasmid-containing C. botulinum type A strains grown in ionic detergent broth and from spontaneously arising variants were screened both for toxin production and for plasmid content. Toxigenicity of C. botulinum could not be correlated with the presence of any one plasmid.
通过对5毫升对数中期培养物细胞的清亮裂解物进行琼脂糖凝胶电泳，对12株A型肉毒梭菌和7株生孢梭菌进行质粒筛选。9株A型菌株有一个或多个大小为4.3、6.8或36兆道尔顿（MDa）的质粒；几株菌株显示有一个61 MDa的大质粒，但并非总能回收得到。4株生孢梭菌菌株有一个或多个大小为4.3、5.6或36 MDa的质粒。从在离子去污剂肉汤中生长的含质粒A型肉毒梭菌培养物以及自发产生的变体中获得的分离株，进行了毒素产生和质粒含量的筛选。肉毒梭菌的产毒性与任何一种质粒的存在均无关联。
