Wangai A W, Pappu S S, Pappu H R, Deom C M, Naidu R A
Department of Plant Pathology.
Department of Entomology.
Plant Dis. 2001 May;85(5):470-474. doi: 10.1094/PDIS.2001.85.5.470.
Groundnut rosette is a major virus disease of peanut in sub-Saharan Africa. The disease is caused by a complex of three agents: GRAV (groundnut rosette assistor luteovirus), GRV (groundnut rosette umbravirus), and the associated satellite RNA (Sat-RNA). During the 1997 to 1998 crop season, the incidence of rosette in farmers' fields was estimated at 24 to 40% in western Kenya and 30% in the Rift Valley. Sequence analysis of Kenyan isolates revealed that GRAV-CP sequences shared 97 to 100% and 95 to 98% sequence homology at nucleotide and amino acid levels, respectively, amongst themselves and with the Malawian and Nigerian isolates. The ORFs 3 and 4 of GRV were similar, with a homology of 99% at the nucleotide and amino acid levels among Kenyan isolates. The GRV sequences of Kenyan isolates were closer to the Malawian (95 to 96%) than to the Nigerian (87 to 88%) isolates. Sat-RNA shared 89 to 94% nucleotide identity with those from Malawi and Nigeria. A closer sequence relationship was observed between Kenyan and Malawian isolates in all regions compared. This is the first report on the distribution and molecular characterization of groundnut rosette disease complex in East Africa.
花生丛簇病是撒哈拉以南非洲地区花生的一种主要病毒病。该病由三种病原体组成的复合体引起：花生丛簇辅助黄症病毒（GRAV）、花生丛簇类病毒（GRV）以及相关的卫星RNA（Sat-RNA）。在1997至1998年作物季，肯尼亚西部农民田地中丛簇病的发病率估计为24%至40%，裂谷地区为30%。对肯尼亚分离株的序列分析表明，GRAV外壳蛋白（CP）序列在核苷酸水平上彼此之间以及与马拉维和尼日利亚分离株的序列同源性分别为97%至100%，在氨基酸水平上为95%至98%。GRV的开放阅读框3和4相似，肯尼亚分离株在核苷酸和氨基酸水平上的同源性为99%。肯尼亚分离株的GRV序列与马拉维分离株（95%至96%）的亲缘关系比与尼日利亚分离株（87%至88%）更近。Sat-RNA与来自马拉维和尼日利亚的Sat-RNA核苷酸同一性为89%至94%。在所比较的所有区域中，肯尼亚和马拉维分离株之间观察到更密切的序列关系。这是关于东非花生丛簇病复合体分布和分子特征的首次报道。
