• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大鼠肝脏微粒体3-羟基-3-甲基戊二酰辅酶A还原酶的特性

Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

作者信息

Ness G C, Sample C E, Smith M, Pendleton L C, Eichler D C

出版信息

Biochem J. 1986 Jan 1;233(1):167-72. doi: 10.1042/bj2330167.

DOI:10.1042/bj2330167
PMID:3082322
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152999/
Abstract

A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an Mr of 100000 was observed. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.

摘要

本文描述了一种制备基本不含溶酶体污染的大鼠肝微粒体组分的方法。当用兔抗大鼠3-羟基-3-甲基戊二酰辅酶A还原酶抗血清通过免疫印迹法检测该制剂时，观察到一条对应于分子量为100000的单带。未发现大鼠肝脏3-羟基-3-甲基戊二酰辅酶A还原酶糖基化的证据。天然大鼠肝微粒体3-羟基-3-甲基戊二酰辅酶A还原酶与纯化的经蛋白水解修饰的物种不同，因为它对NADPH表现出别构动力学。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/1152999/b2b1ccf357e5/biochemj00288-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/1152999/df64e83741ef/biochemj00288-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/1152999/e9e9fc5bed70/biochemj00288-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/1152999/b2b1ccf357e5/biochemj00288-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/1152999/df64e83741ef/biochemj00288-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/1152999/e9e9fc5bed70/biochemj00288-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/1152999/b2b1ccf357e5/biochemj00288-0174-b.jpg

相似文献

1
Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase.大鼠肝脏微粒体3-羟基-3-甲基戊二酰辅酶A还原酶的特性
Biochem J. 1986 Jan 1;233(1):167-72. doi: 10.1042/bj2330167.
2
Activation of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase by NADPH. Effects of dietary treatments.
J Biol Chem. 1985 Oct 15;260(23):12391-3.
3
Altered kinetic properties of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase following dietary manipulations.饮食干预后大鼠肝脏3-羟基-3-甲基戊二酰辅酶A还原酶的动力学特性改变
J Biol Chem. 1986 Apr 15;261(11):5061-6.
4
The effects of NADPH and 3-hydroxy-3-methylglutaryl-CoA on the thiol/disulfide redox behavior of rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase.
J Biol Chem. 1990 Sep 15;265(26):15464-70.
5
Diurnal changes in the fraction of 3-hydroxy-3-methylglutaryl-CoA reductase in the active form in rat liver microsomal fractions.大鼠肝脏微粒体组分中活性形式的3-羟基-3-甲基戊二酰辅酶A还原酶比例的昼夜变化。
Biochem J. 1984 Jun 15;220(3):739-45. doi: 10.1042/bj2200739.
6
Solubilization of the 97-kDa native form of liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase.肝脏微粒体3-羟基-3-甲基戊二酰辅酶A还原酶97-kDa天然形式的增溶作用。
J Biol Chem. 1990 Jun 15;265(17):9984-92.
7
Modulation of 3-hydroxy-3-methylglutaryl-CoA reductase and of acyl-CoA--cholesterol acyltransferase by the transfer of non-esterified cholesterol to rat liver microsomal vesicles.通过将非酯化胆固醇转移至大鼠肝微粒体囊泡对3-羟基-3-甲基戊二酰辅酶A还原酶和酰基辅酶A-胆固醇酰基转移酶的调节
Biochem J. 1981 Jan 15;194(1):265-71. doi: 10.1042/bj1940265.
8
Influence of a naturally occurring competing enzymic activity on studies of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity.
Biochim Biophys Acta. 1979 Feb 26;572(2):333-44. doi: 10.1016/0005-2760(79)90049-3.
9
Sulfhydryl/disulfide forms of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase.大鼠肝脏3-羟基-3-甲基戊二酰辅酶A还原酶的巯基/二硫键形式
J Biol Chem. 1985 Dec 25;260(30):16395-9.
10
Regulation of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase. Evidence for thiol-dependent allosteric modulation of enzyme activity.大鼠肝脏3-羟基-3-甲基戊二酰辅酶A还原酶的调节。酶活性的硫醇依赖性变构调节的证据。
J Biol Chem. 1984 Jan 25;259(2):870-7.

引用本文的文献

1
A critical role of mevalonate for peptidoglycan synthesis in Staphylococcus aureus.甲羟戊酸在金黄色葡萄球菌肽聚糖合成中的关键作用。
Sci Rep. 2016 Mar 10;6:22894. doi: 10.1038/srep22894.
2
Identification of Novel Tau Interactions with Endoplasmic Reticulum Proteins in Alzheimer's Disease Brain.阿尔茨海默病大脑中与内质网蛋白相互作用的新型tau蛋白的鉴定
J Alzheimers Dis. 2015;48(3):687-702. doi: 10.3233/JAD-150298.
3
Deciphering molecular mechanism underlying hypolipidemic activity of echinocystic Acid.解析山楂酸降血脂活性的分子机制。

本文引用的文献

1
Proteinase involvement in the solubilization of 3-hydroxy-3-methylglutaryl coenzyme A reductase.蛋白酶在3-羟基-3-甲基戊二酰辅酶A还原酶溶解过程中的作用。
Biochem Biophys Res Commun. 1981 Sep 16;102(1):81-5. doi: 10.1016/0006-291x(81)91491-1.
2
Molecular cloning of 3-hydroxy-3-methylglutaryl coenzyme a reductase and evidence for regulation of its mRNA.3-羟基-3-甲基戊二酰辅酶A还原酶的分子克隆及其mRNA调控的证据
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7704-8. doi: 10.1073/pnas.79.24.7704.
3
Appearance of crystalloid endoplasmic reticulum in compactin-resistant Chinese hamster cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
Evid Based Complement Alternat Med. 2014;2014:823154. doi: 10.1155/2014/823154. Epub 2014 Feb 11.
4
Mechanism of resistance to dietary cholesterol.对膳食胆固醇的抗性机制。
J Lipids. 2011;2011:101242. doi: 10.1155/2011/101242. Epub 2011 Oct 5.
5
LDLR expression and localization are altered in mouse and human cell culture models of Alzheimer's disease.阿尔茨海默病的小鼠和人细胞培养模型中 LDLR 的表达和定位发生改变。
PLoS One. 2010 Jan 1;5(1):e8556. doi: 10.1371/journal.pone.0008556.
6
Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs.硫酯酶超家族成员2（Them2）/酰基辅酶A硫酯酶13（Acot13）：一种对长链脂肪酰基辅酶A具有选择性的同四聚体热狗折叠硫酯酶。
Biochem J. 2009 Jun 26;421(2):311-22. doi: 10.1042/BJ20090039.
7
7-Dehydrocholesterol-dependent proteolysis of HMG-CoA reductase suppresses sterol biosynthesis in a mouse model of Smith-Lemli-Opitz/RSH syndrome.在史密斯-勒米-奥皮茨/RSH综合征小鼠模型中，7-脱氢胆固醇依赖性的HMG-CoA还原酶蛋白水解作用抑制了甾醇生物合成。
J Clin Invest. 2001 Sep;108(6):905-15. doi: 10.1172/JCI12103.
8
Enhancement of sterol synthesis by the monoterpene perillyl alcohol is unaffected by competitive 3-hydroxy-3-methylglutaryl-CoA reductase inhibition.单萜紫苏醇对甾醇合成的增强作用不受竞争性3-羟基-3-甲基戊二酰辅酶A还原酶抑制的影响。
Lipids. 1999 Jun;34(6):605-15. doi: 10.1007/s11745-999-0405-5.
9
Loss of NADPH during assays of HMG-CoA reductase: implications and approaches to minimize errors.
Lipids. 1987 Jun;22(6):409-12. doi: 10.1007/BF02537269.
10
In vivo regulation of human mononuclear leukocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase. Decreased enzyme catalytic efficiency in familial hypercholesterolemia.人单核白细胞3-羟基-3-甲基戊二酰辅酶A还原酶的体内调节。家族性高胆固醇血症中酶催化效率降低。
J Clin Invest. 1987 Nov;80(5):1401-8. doi: 10.1172/JCI113218.
在对康帕丁有抗性的中国仓鼠细胞中出现晶体样内质网，其3-羟基-3-甲基戊二酰辅酶A还原酶增加了500倍。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1185-9. doi: 10.1073/pnas.79.4.1185.
4
Amplification of the gene for 3-hydroxy-3-methylglutaryl coenzyme A reductase, but not for the 53-kDa protein, in UT-1 cells.在UT-1细胞中，3-羟基-3-甲基戊二酰辅酶A还原酶基因发生扩增，但53 kDa蛋白的基因未扩增。
J Biol Chem. 1983 Jul 10;258(13):8462-9.
5
Mevalonolactone inhibits the rate of synthesis and enhances the rate of degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in rat hepatocytes.甲羟戊酸内酯抑制大鼠肝细胞中3-羟基-3-甲基戊二酰辅酶A还原酶的合成速率并提高其降解速率。
J Biol Chem. 1983 Jun 25;258(12):7272-5.
6
Topography of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Biochem Biophys Res Commun. 1984 Mar 15;119(2):772-8. doi: 10.1016/s0006-291x(84)80317-4.
7
Regulation of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase. Evidence for thiol-dependent allosteric modulation of enzyme activity.大鼠肝脏3-羟基-3-甲基戊二酰辅酶A还原酶的调节。酶活性的硫醇依赖性变构调节的证据。
J Biol Chem. 1984 Jan 25;259(2):870-7.
8
Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe.用单克隆抗体和cDNA探针研究大鼠肝脏中3-羟基-3-甲基戊二酰辅酶A还原酶及其mRNA的调控。
J Biol Chem. 1983 Jul 10;258(13):8450-5.
9
Overproduction of a Mr 92,000 protomer of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in compactin-resistant C100 cells.在对美伐他汀耐药的C100细胞中，3-羟基-3-甲基戊二酰辅酶A还原酶的一种分子量为92,000的亚基过度产生。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1516-20. doi: 10.1073/pnas.80.6.1516.
10
Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum.3-羟基-3-甲基戊二酰辅酶A还原酶的核苷酸序列，一种内质网糖蛋白。
Nature. 1984;308(5960):613-7. doi: 10.1038/308613a0.