大鼠肝脏微粒体3-羟基-3-甲基戊二酰辅酶A还原酶的特性
Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
作者信息
Ness G C, Sample C E, Smith M, Pendleton L C, Eichler D C
出版信息
Biochem J. 1986 Jan 1;233(1):167-72. doi: 10.1042/bj2330167.
Abstract
A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an Mr of 100000 was observed. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.
摘要
本文描述了一种制备基本不含溶酶体污染的大鼠肝微粒体组分的方法。当用兔抗大鼠3-羟基-3-甲基戊二酰辅酶A还原酶抗血清通过免疫印迹法检测该制剂时，观察到一条对应于分子量为100000的单带。未发现大鼠肝脏3-羟基-3-甲基戊二酰辅酶A还原酶糖基化的证据。天然大鼠肝微粒体3-羟基-3-甲基戊二酰辅酶A还原酶与纯化的经蛋白水解修饰的物种不同，因为它对NADPH表现出别构动力学。
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