Sorbonne Université, UMR7622-IBPS, Paris, France.
Univ Paris Diderot, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Paris, France.
Plant Sci. 2019 Mar;280:408-415. doi: 10.1016/j.plantsci.2018.12.015. Epub 2018 Dec 18.
Using various inhibitors and scavengers we took advantage of the size of sunflower (Helianthus annuus) seeds to investigate in vivo the effects of hormones, namely abscisic acid (ABA) and ethylene (ET), and reactive oxygen species (ROS) on the polarization of dormant (D) and non-dormant (ND) embryonic seed cells using microelectrodes. Our data show that D and ND seed cells present different polarization likely due to the regulation of plasma membrane (PM) H-ATPase activity. The data obtained after addition of hormones or ROS scavengers further suggest that ABA dependent inhibition of PM H-ATPases could participate in dormancy maintenance and that ET-and ROS-dependent PM H-ATPase stimulation could participate in dormancy release in sunflower seeds.
利用各种抑制剂和清除剂，我们利用向日葵（Helianthus annuus）种子的大小，利用微电极在体内研究激素（即脱落酸（ABA）和乙烯（ET））和活性氧（ROS）对休眠（D）和非休眠（ND）胚胎种子细胞极化的影响。我们的数据表明，D 和 ND 种子细胞呈现出不同的极化，这可能是由于质膜（PM）H-ATP 酶活性的调节。添加激素或 ROS 清除剂后获得的数据进一步表明，ABA 依赖性 PM H-ATP 酶抑制可能参与休眠维持，而 ET 和 ROS 依赖性 PM H-ATP 酶刺激可能参与向日葵种子休眠的释放。
