Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Crop Science Department, University of Zimbabwe, Harare 00263, Zimbabwe.
Genes (Basel). 2020 Mar 27;11(4):361. doi: 10.3390/genes11040361.
The P-type plasma membrane (PM) H-ATPase plays a major role during the growth and development of a plant. It is also involved in plant resistance to a variety of biotic and abiotic factors, including salt stress. The PM H-ATPase gene family has been well characterized in and other crop plants such as rice, cucumber, and potato; however, the same cannot be said in sunflower (). In this study, a total of thirteen PM H-ATPase genes were screened from the recently released sunflower genome database with a comprehensive genome-wide analysis. According to a systematic phylogenetic classification with a previously reported species, the sunflower PM H-ATPase genes () were divided into four sub-clusters (I, II, IV, and V). In addition, systematic bioinformatics analyses such as gene structure analysis, chromosome location analysis, subcellular localization predication, conserved motifs, and -acting elements of promoter identification were also done. Semi-quantitative PCR analysis data of in different sunflower tissues revealed the specificity of gene spatiotemporal expression and sub-cluster grouping. Those belonging to sub-cluster I and II exhibited wide expression in almost all of the tissues studied while sub-cluster IV and V seldom showed expression. In addition, the expression of , and was shown to be induced by salt stress. The transgenic plants overexpressing and showed higher salinity tolerance compared with wild-type plants. Further analysis showed that the Na content of transgenic plants decreased under salt stress, which indicates that PM H ATPase participates in the physiological process of Na efflux, resulting in salt resistance of the plants. This study is the first to identify and analyze the sunflower PM H ATPase gene family. It does not only lay foundation for future research but also demonstrates the role played by in salt stress tolerance.
质膜 P 型 H+-ATP 酶在植物生长发育过程中起着重要作用。它还参与植物对各种生物和非生物因素的抗性，包括盐胁迫。质膜 H+-ATP 酶基因家族在拟南芥和其他作物如水稻、黄瓜和土豆中得到了很好的描述；然而，在向日葵中却并非如此。在这项研究中，从最近发布的向日葵基因组数据库中筛选出了 13 个质膜 H+-ATP 酶基因，并进行了全面的全基因组分析。根据与先前报道的物种进行的系统系统发育分类，向日葵质膜 H+-ATP 酶基因（）分为四个亚群（I、II、IV 和 V）。此外，还进行了系统的生物信息学分析，如基因结构分析、染色体定位分析、亚细胞定位预测、保守基序和启动子识别的顺式作用元件分析。在不同向日葵组织中进行的半定量 PCR 分析数据表明，基因时空表达的特异性和亚群分组。属于亚群 I 和 II 的基因在几乎所有研究的组织中都有广泛的表达，而亚群 IV 和 V 则很少表达。此外，盐胁迫诱导了 、 和 的表达。过表达 和 的转基因植物与野生型植物相比表现出更高的耐盐性。进一步的分析表明，在盐胁迫下，转基因 植物的 Na 含量降低，这表明质膜 H+-ATP 酶参与了 Na 外排的生理过程，从而提高了植物的耐盐性。这项研究首次鉴定和分析了向日葵质膜 H+-ATP 酶基因家族。它不仅为未来的研究奠定了基础，还证明了 在耐盐性中的作用。
