State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Science, Henan University, Kaifeng, Henan 475004, PR China.
State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Science, Henan University, Kaifeng, Henan 475004, PR China.
Plant Sci. 2019 Apr;281:72-81. doi: 10.1016/j.plantsci.2019.01.012. Epub 2019 Jan 18.
Salinity is a major abiotic stress limiting plant growth and development that has caused severe damage to yield and quality of cotton fiber. Uncovering the mechanisms of response to salt stress is important in breeding salt-tolerant cotton varieties. Transcriptome analysis identified 2356 differentially expressed genes in cotton under salt stress, of which 9.4% were predicted transcription factors (TFs). Approximately 17.6% (39 out of 222) of the differentially expressed TFs belonged to the ethylene response factor (ERF) family. Expression pattern analysis showed significant changes in these ERFs during salt stress. Moreover, the number of down-regulated ERFs was more than that of the up-regulated ERFs. Two of the ERFs, GhERF4L and GhERF54L, showed increased (12-15 times) expression after 12 h of salt treatment. Silencing of GhERF4L and GhERF54L significantly reduced salt tolerance of cotton seedlings, indicating their role in regulating cotton response to salt stress. This study revealed the essential role of ERF transcription factors in the salt response mechanism of plants, and provided important genetic resources for breeding salt-tolerant cotton.
盐度是限制植物生长和发育的主要非生物胁迫因素，它对棉花纤维的产量和质量造成了严重损害。揭示植物对盐胁迫的响应机制对于培育耐盐棉花品种非常重要。转录组分析鉴定出盐胁迫下棉花中 2356 个差异表达基因，其中 9.4%被预测为转录因子（TFs）。在差异表达的 TFs 中，大约有 17.6%（39/222）属于乙烯响应因子（ERF）家族。表达模式分析表明，这些 ERFs 在盐胁迫下发生了显著变化。此外，下调的 ERFs 数量多于上调的 ERFs。两个 ERFs，GhERF4L 和 GhERF54L，在盐处理 12 小时后表达水平增加了（12-15 倍）。GhERF4L 和 GhERF54L 的沉默显著降低了棉花幼苗的耐盐性，表明它们在调节棉花对盐胁迫的响应中起作用。本研究揭示了 ERF 转录因子在植物盐响应机制中的重要作用，为培育耐盐棉花提供了重要的遗传资源。
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