Zhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Biomolecules. 2023 Sep 5;13(9):1348. doi: 10.3390/biom13091348.
wilt is a fungal disease in upland cotton and exerts a significant effect on growth and potential productivity. This disease is mainly caused by Kleb. Ethylene response factor (ERF) is one of the superfamilies of transcription factors that is involved in the development and environmental adaption of crops. A total of 30 ERF.B4 group members were detected in upland cotton and divided into 6 subgroups. Gene structures, conserved motifs, and domain analysis revealed that members in each subgroup are highly conserved. Further, the 30 GhERF.B4 group members were distributed on 18 chromosomes, and 36 gene synteny relationships were found among them. genes were ubiquitously expressed in various tissues and developmental stages of cotton. Amongst them, was predominantly expressed in roots, and its expression was induced by infection. In addition, responded to methyl jasmonate (MeJA), methyl salicylate (MeSA), and ethylene (ET) phytohormones. It was also found that the resistance was enhanced due to overexpression of in . On the contrary, interference of by virus-induced gene silencing (VIGS) technology decreased the resistance level in upland cotton. The subcellular localization experiment showed that GhERF.B4-15D was located in the nucleus. Yeast two-hybrid (Y2H) and luciferase complementation (LUC) approaches demonstrated that GhERF.B4-15D interacted with GhDREB1B. Additionally, the resistance was significantly decreased in GhDREB1B knockdowns. Our results showed that GhERF.B4-15D plays a role during infection in cotton.
枯萎病是一种棉花真菌病害，对棉花的生长和潜在生产力有显著影响。这种疾病主要是由 Kleb 引起的。乙烯响应因子（ERF）是转录因子的超家族之一，参与作物的发育和环境适应。在陆地棉中总共检测到 30 个 ERF.B4 组成员，并分为 6 个亚组。基因结构、保守基序和结构域分析表明，每个亚组的成员高度保守。此外，30 个 GhERF.B4 组成员分布在 18 条染色体上，发现它们之间存在 36 个基因同线性关系。GhERF.B4 基因在棉花的各种组织和发育阶段普遍表达。其中，GhERF.B4-15D 在根中表达量最高，其表达受 感染诱导。此外，GhERF.B4-15D 对茉莉酸甲酯（MeJA）、水杨酸甲酯（MeSA）和乙烯（ET）植物激素有反应。还发现，由于 过表达， 增强了对 的抗性。相反，通过病毒诱导的基因沉默（VIGS）技术干扰 ，降低了陆地棉的 抗性水平。亚细胞定位实验表明 GhERF.B4-15D 位于细胞核中。酵母双杂交（Y2H）和荧光素酶互补（LUC）实验表明 GhERF.B4-15D 与 GhDREB1B 相互作用。此外，GhDREB1B 敲低后， 的抗性显著降低。我们的研究结果表明，GhERF.B4-15D 在棉花的 感染过程中发挥作用。
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