Wang Yuting, Ally Msangi Shamsia, Wang Ruiqi, Yao Wenjing, Jiang Tingbo, Liu Huanzhen
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Hexing Road, Harbin 150040, China.
Bamboo Research Institute, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.
Plants (Basel). 2025 Aug 27;14(17):2681. doi: 10.3390/plants14172681.
Drought and salinity are two major environmental factors that severely limit plant growth and development. MYB functions as a transcription factor that is crucial in how plants respond to stress from adverse conditions. In this study, we identified a gene encoding MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor, whose transcription level was significantly induced under salt stress and osmotic stress. Subcellular localisation results showed was located in the nucleus. Yeast one-hybrid assay indicated the gene exhibited transcriptional activation activity and it can precisely bind to the G-box elements. Under normal growth conditions, there were no significant differences in physiological and biochemical indicators between wild-type and transgenic tobacco. However, under salt and drought stress, transgenic tobacco overexpressing exhibited superior root length and fresh weight compared to the wild-type (WT), with higher levels of SOD, POD, proline, and chlorophyll content, and significantly lower MDA and HO content than the WT. These findings indicate that significantly enhances the salt tolerance and drought resistance of transgenic tobacco. These results indicate that PsnMYB30 is a key target gene for studying salt-tolerant and drought-resistant plants in genetic breeding.
干旱和盐度是严重限制植物生长发育的两个主要环境因素。MYB作为一种转录因子，在植物应对逆境胁迫的过程中起着关键作用。在本研究中，我们鉴定了一个编码MYB（v-myb禽成髓细胞瘤病毒癌基因同源物）转录因子的基因，其转录水平在盐胁迫和渗透胁迫下显著上调。亚细胞定位结果表明该基因定位于细胞核。酵母单杂交试验表明该基因具有转录激活活性，并且能够精确结合G-box元件。在正常生长条件下，野生型和转基因烟草的生理生化指标没有显著差异。然而，在盐胁迫和干旱胁迫下，过表达该基因的转基因烟草与野生型相比，根长和鲜重表现更优，超氧化物歧化酶（SOD）、过氧化物酶（POD）、脯氨酸和叶绿素含量更高，丙二醛（MDA）和过氧化氢（H₂O₂）含量显著低于野生型。这些结果表明该基因显著增强了转基因烟草的耐盐性和抗旱性。这些结果表明PsnMYB30是遗传育种中研究耐盐抗旱植物的关键靶基因。
