用于体内血管生成评估的基质胶栓试验
Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis.
作者信息
Kastana Pinelopi, Zahra Fatema Tuz, Ntenekou Despoina, Katraki-Pavlou Stamatiki, Beis Dimitris, Lionakis Michail S, Mikelis Constantinos M, Papadimitriou Evangelia
机构信息
Laboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
出版信息
Methods Mol Biol. 2019;1952:219-232. doi: 10.1007/978-1-4939-9133-4_18.
Matrigel is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma in C57BL/6 mice, a tumor rich in extracellular matrix (ECM) proteins. It consists mainly of laminin (approximately 60%), collagen IV (approximately 30%), and nidogen-1/entactin (approximately 8%), while it also contains heparan sulfate proteoglycans, such as perlecan, other ECM proteins, as well as growth factors bound to the ECM. Matrigel mimics the physiological cell matrix and is the most commonly used matrix substrate to study in vitro and in vivo angiogenesis. Here, we describe the in vivo Matrigel plug assay and how it can be used for both qualitative and quantitative assessment of angiogenesis.
基质胶是从C57BL/6小鼠的恩格尔布雷特-霍尔姆-斯旺（EHS）小鼠肉瘤中提取的，该肿瘤富含细胞外基质（ECM）蛋白。它主要由层粘连蛋白（约60%）、IV型胶原蛋白（约30%）和巢蛋白-1/巢丝蛋白（约8%）组成，同时还含有硫酸乙酰肝素蛋白聚糖，如基底膜聚糖、其他ECM蛋白以及与ECM结合的生长因子。基质胶模拟生理细胞基质，是研究体外和体内血管生成最常用的基质底物。在此，我们描述了体内基质胶栓试验及其如何用于血管生成的定性和定量评估。
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