Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil.
Hospital Israelita Albert Einstein, São Paulo, Brazil.
J Neurosci Res. 2019 Jul;97(7):760-771. doi: 10.1002/jnr.24399. Epub 2019 Mar 2.
Immediate early genes (IEGs) are a fundamental element in the way we respond and adapt to a variety of stimuli. We have recently reported that IEG response, as measured by c-Fos expression, is different between rodents and primates. Here, we further extend this analysis by assessing the expression of c-Jun, one of the main complements of c-Fos, under the same stimulation protocol. For this, we investigated the immunohistochemical expression of c-Jun (and compared with that previously shown for c-Fos) after stimulation with pentylenetetrazol in the cingulate gyrus, motor cortex, piriform cortex, inferior temporal cortex, and visual cortex of rats and marmosets (Callithrix jacchus), both male and female. Overall the immunohistochemical expression of c-Jun was more intense but remained elevated for a shorter duration in marmosets as compared to rats. These results are in contrast to what we had previously shown for c-Fos. Furthermore, in terms of the temporal profile, c-Fos and c-Jun expression occurred in a complementary manner in rats-the peak of c-Fos expression coincided with low levels of c-jun expression-and in a superimposed manner in marmosets-the peak of c-Fos expression coincided with the peak of c-Jun expression. Since Fos proteins may form dimers with Jun proteins and together control late gene expressions in the cell nucleus, this different expression profile between primates and rodents may bear meaningful impact for how the nervous system reacts and adapts to stimulation.
立即早期基因（IEGs）是我们对各种刺激做出反应和适应的基本要素。我们最近报告称，IEG 反应（通过 c-Fos 表达来衡量）在啮齿动物和灵长类动物之间存在差异。在这里，我们通过评估相同刺激方案下 c-Jun 的表达，进一步扩展了这一分析。为此，我们研究了戊四氮刺激后，大鼠和狨猴（Callithrix jacchus）（雄性和雌性）扣带回皮质、运动皮质、梨状皮质、下颞叶皮质和视皮质中 c-Jun 的免疫组织化学表达（并与之前显示的 c-Fos 进行了比较）。总的来说，与大鼠相比，狨猴的 c-Jun 免疫组织化学表达更强烈，但持续时间更短。这些结果与我们之前对 c-Fos 的研究结果相反。此外，就时间分布而言，c-Fos 和 c-Jun 的表达在大鼠中以互补的方式发生——c-Fos 表达的峰值与 c-jun 表达的低水平相对应——而在狨猴中则以叠加的方式发生——c-Fos 表达的峰值与 c-Jun 表达的峰值相对应。由于 Fos 蛋白可能与 Jun 蛋白形成二聚体，并共同控制细胞核中晚期基因的表达，因此灵长类动物和啮齿动物之间这种不同的表达模式可能对神经系统对刺激的反应和适应方式产生有意义的影响。
