Beckmann A M, Matsumoto I, Wilce P A
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Neuropharmacology. 1995 Sep;34(9):1183-9. doi: 10.1016/0028-3908(95)00089-o.
The expression of immediate early genes (IEG)s c-fos, c-jun and zif/268 was studied during naloxone-precipitated morphine withdrawal in various organs of the rat. Dependence was induced over a period of 6 days by a graded regimen of 6-hourly injections. Northern analysis revealed peak expression of all IEGs occurred in the forebrain plus cerebellum at 20 min and at 60 min in the brain stem following morphine withdrawal. Increased levels of c-fos and c-jun mRNA were observed in the spinal cord at 40 min of morphine withdrawal. An increase in c-fos and c-jun but not zif/268 mRNAs was seen in the jejunum between 20 and 60 min. Elevated levels of the IEG protein products in the cerebral cortex, hippocampus, thalamus, cerebellum, brain stem and spinal cord were observed at 60 min following morphine withdrawal. These data emphasize the temporal and spatial variation in IEG expression in different tissues during opiate withdrawal.
在大鼠的纳洛酮诱发吗啡戒断过程中，研究了即刻早期基因（IEG）c-fos、c-jun和zif/268在大鼠各器官中的表达。通过每6小时注射一次的分级给药方案，在6天的时间里诱导大鼠对吗啡产生依赖。Northern分析显示，所有IEG的表达峰值在吗啡戒断后20分钟出现在前脑加小脑，在脑干则在60分钟出现。在吗啡戒断40分钟时，脊髓中c-fos和c-jun mRNA水平升高。在20至60分钟之间，空肠中c-fos和c-jun mRNA增加，但zif/268 mRNA未见增加。在吗啡戒断60分钟后，观察到大脑皮层、海马体、丘脑、小脑、脑干和脊髓中IEG蛋白产物水平升高。这些数据强调了阿片类药物戒断期间不同组织中IEG表达的时空变化。
