School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK.
George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia.
J Appl Microbiol. 2019 Jun;126(6):1657-1667. doi: 10.1111/jam.14241.
This paper presents the potential of environmentally sourced bacteriophages to affect the growth of clinical isolates of Pseudomonas aeruginosa biofilms, and assesses the respective plaque morphotypes presented by each bacteriophage, in vitro.
Bacterial host strains were typed for their ability to produce the quorum sensing-controlled virulence factor pyocyanin, and then tested for bacteriophage susceptibility using the spot test method. The bacteriophages were co-administered with ciprofloxacin in order to determine whether the bacteriophages would demonstrate synergistic or antagonistic behaviour to the antibiotic in vitro. Results suggest a potential relationship between the bacteriophage plaque size and biofilm inhibition, where those producing smaller plaques appear to be more effective at reducing bacterial biofilm formation.
This phenomenon may be explained by a high adsorption rate leading to the rapid formation of smaller plaques, and greater biofilm reduction associated with the loss of viable bacterial cells before the cells can adhere to the surface and form a biofilm. Results from the co-administration of bacteriophage and ciprofloxacin suggest that the two work synergistically to affect P. aeruginosa biofilms.
The data indicate enhanced efficacy of ciprofloxacin by ≥50%. This could offer an alternative strategy for targeting antibiotic-resistant infections.
本文旨在探讨环境来源的噬菌体对铜绿假单胞菌生物膜临床分离株生长的影响，并评估每种噬菌体在体外形成的菌斑形态。
对细菌宿主菌株进行产群体感应控制的毒力因子绿脓菌素的能力分型，然后使用点测试法测试噬菌体的敏感性。将噬菌体与环丙沙星联合使用，以确定噬菌体在体外对抗生素是否表现出协同或拮抗作用。结果表明，噬菌体菌斑大小与生物膜抑制之间存在潜在关系，其中产生较小菌斑的噬菌体似乎更能有效减少细菌生物膜的形成。
这种现象可能是由于高吸附率导致快速形成较小的菌斑，以及在细菌细胞能够附着到表面并形成生物膜之前，由于活细菌细胞的丧失，导致更大的生物膜减少。噬菌体和环丙沙星联合给药的结果表明，两者协同作用以影响铜绿假单胞菌生物膜。
数据表明环丙沙星的疗效提高了≥50%。这为靶向抗生素耐药感染提供了一种替代策略。
