从长江三角洲白山羊新生个体中分离得到的毛囊干细胞。

Hair follicle stem cells isolated from newborn Yangtze River Delta White Goats.

机构信息

Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

出版信息

Gene. 2019 May 25;698:19-26. doi: 10.1016/j.gene.2019.02.052. Epub 2019 Feb 27.

DOI:10.1016/j.gene.2019.02.052

PMID:30825596

Abstract

Adult stem cells are self-renewing populations that originate from embryonic progenitor cells during organogenesis and retain multipotency to support tissue and organ regeneration throughout the lifetime of an organism. The hair follicle (HF) is a small organ that is ideal for studying the biology and regulation of adult stem cells. A distinct, permanent pool of adult stem cells is located in the HF bulge region. Most methods used to isolate hair follicle stem cells (HFSCs) begin with mouse or human follicles. Here, we describe two methods of isolating HFSCs from newborn Yangtze River Delta White Goats. A suitable method was found. The cell viability and expression of HFSC marker proteins differed in the two methods. CD49f-positive (integrin alpha 6) HFSCs were sorted by fluorescence activated flow cytometry. Sorted HFSCs can be used in various in vivo grafting models and are useful as an in vitro model to study multipotency, quiescence and activation.

摘要

成体干细胞是自我更新的细胞群体，来源于器官发生过程中的胚胎祖细胞，并保持多能性，以支持生物体内组织和器官的再生。毛囊（HF）是一个小器官，非常适合研究成体干细胞的生物学和调控。一个独特的、永久的成体干细胞池位于 HF 隆起区域。大多数用于分离毛囊干细胞（HFSCs）的方法都是从小鼠或人类的毛囊开始的。在这里，我们描述了从新生的长三角白山羊中分离 HFSCs 的两种方法。找到了一种合适的方法。两种方法分离的 HFSC 细胞活力和 HFSC 标记蛋白的表达不同。通过荧光激活流式细胞术对 CD49f 阳性（整合素α6）HFSCs 进行分选。分选的 HFSCs 可用于各种体内移植模型，并且可作为体外模型用于研究多能性、静止和激活。