Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2022 Oct 28;13(11):1973. doi: 10.3390/genes13111973.
Yangtze River Delta white goats are the sole goat breed producing brush hair of high quality. The gene has been extensively studied in tumor cells but rarely in hair follicle stem cells (HFSCs). Per the previous sequencing data, it was determined that expression was up-regulated in superior-quality brush hair tissues, confirming it as a candidate gene associated with this trait. The targeting relationship of miR-145-5p with was determined based on online database prediction and was authenticated using a dual-luciferase gene reporter assay and quantitative reverse-transcription PCR (RT-qPCR). The regulatory effect of miR-145-5p on the growth of HFSCs was determined by targeting with RT-qPCR, 5-ethynyl-2'-deoxyuridine assays, Western blotting, and flow cytometry. The proliferation of HFSCs was inhibited and their apoptosis capacity was enhanced due to the presence of miR-145-5p. Therefore, it was proposed that this may have occurred through a repression effect of on the MAPK signaling pathway. The regulatory network of the HFSCs can be further understood using the theoretical basis established by the findings derived from this study.
长江三角洲白山羊是唯一产优质刷毛的山羊品种。该基因已在肿瘤细胞中进行了广泛研究，但在毛囊干细胞（HFSCs）中很少研究。根据之前的测序数据，确定在优质刷毛组织中上调表达，证实其为与该性状相关的候选基因。通过在线数据库预测确定了 miR-145-5p 与的靶向关系，并通过双荧光素酶基因报告基因测定和定量逆转录 PCR（RT-qPCR）进行了验证。通过 RT-qPCR、5-乙炔基-2'-脱氧尿苷测定、Western blot 和流式细胞术确定了 miR-145-5p 对 HFSCs 生长的靶向调控作用。由于存在 miR-145-5p，HFSCs 的增殖受到抑制，凋亡能力增强。因此，提出这可能是通过抑制对 MAPK 信号通路的作用来实现的。本研究的发现为 HFSCs 的调控网络提供了理论基础。
