Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China.
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China.
J Pharm Biomed Anal. 2019 May 10;168:189-200. doi: 10.1016/j.jpba.2019.02.027. Epub 2019 Feb 19.
Natural products (NPs) have a long history of clinical use and are rich source of bioactive compounds. The development of tools and techniques for identifying and analyzing NP bioactive compounds to ensure their quality and discover new drugs is thus very important and still in demand. Screening techniques have proven highly useful for screening and analyzing active components in complex mixtures, which rely on cell culture, dialysis, ultrafiltration, chromatographic methods and target molecule immobilization, using biological targets to identify the active compounds. The recent progress in biological screening techniques in the field of natural products is reviewed here. This includes a review on the strategy and application of the screening methods, their detailed description and discussion of their existing limitations of the different models along with prospective in future development of screening techniques.
天然产物（NPs）在临床应用中有着悠久的历史，是生物活性化合物的丰富来源。因此，开发用于鉴定和分析 NP 生物活性化合物的工具和技术以确保其质量并发现新药非常重要且仍有需求。筛选技术已被证明对筛选和分析复杂混合物中的活性成分非常有用，这些技术依赖于细胞培养、透析、超滤、色谱方法和靶分子固定化，使用生物靶标来鉴定活性化合物。本文综述了天然产物领域生物筛选技术的最新进展。这包括对筛选方法的策略和应用的回顾，对不同模型的详细描述和讨论，以及对未来筛选技术发展的展望。
