College of Pharmaceutical Sciences, Fuchun Campus, Zhejiang Chinese Medical University, Hangzhou, China.
Biomed Chromatogr. 2022 Oct;36(10):e5447. doi: 10.1002/bmc.5447. Epub 2022 Aug 10.
Natural products (NP) are an important source of bioactive compounds. Considering their complex matrix effects, the development of suitable methodologies for the quick identification and analysis of active substances in NPs played a significant role in controlling their quality and discovering new drugs. In recent years, the technology of immobilized biomembrane has attracted increasing attention, due to its advantages such as multitarget efficiency, accuracy, and/or time-saving compared with traditional activity-guided separation and ligand fishing methods. This article provides a systematic review of the latest advances in screening technologies based on biomembrane in the field of NPs. It includes detailed discussions on these technologies, including cell membrane chromatography, artificial membrane chromatography, cell membrane fishing, living cell fishing methods, and their applications in screening various active molecules from NPs. Their limitations and future development prospects are further discussed.
天然产物（NP）是生物活性化合物的重要来源。考虑到它们复杂的基质效应，开发合适的方法来快速鉴定和分析 NP 中的活性物质，对于控制其质量和发现新药具有重要意义。近年来，固定化生物膜技术因其具有比传统的活性导向分离和配体钓取方法更高效、更准确和/或更省时的特点，引起了越来越多的关注。本文对基于 NP 中生物膜的筛选技术的最新进展进行了系统综述。详细讨论了这些技术，包括细胞膜色谱法、人工膜色谱法、细胞膜钓取法、活细胞钓取法及其在筛选 NP 中各种活性分子方面的应用。进一步讨论了它们的局限性和未来的发展前景。
