Department of Dermatology and Venereology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-413 45 Gothenburg, Sweden.
Department of Dermatology and Venereology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-413 45 Gothenburg, Sweden.
J Steroid Biochem Mol Biol. 2019 May;189:154-160. doi: 10.1016/j.jsbmb.2019.02.015. Epub 2019 Feb 28.
Recently it has been recognized that there is a need of investigating in detail the vitamin D synthesis and metabolism directly in the skin with respect to its possible autocrine and paracrine actions. The potential effects the active metabolite of vitamin D exerts in pathological skin conditions like psoriasis needs to be clarified. Under ultraviolet B (UVB) irradiation skin can autonomously synthesize, activate and degrade vitamin D. In this pilot case study, we used for the first time Time-of-flight secondary ion mass spectrometry (ToF-SIMS) in the analysis of skin biopsies from a patient with psoriasis before and after UVB phototherapy. We were able to visualize vitamin D and its metabolites in the skin and subcutaneous tissue. At the same time information about their localization at subcellular level and morphology of the skin was received. This study proves that ToF-SIMS is a promising powerful tool to be used when investigating vitamin D´s role in dermatological diseases through skin biopsies.
最近已经认识到，需要详细研究皮肤中维生素 D 的合成和代谢，以了解其可能的自分泌和旁分泌作用。需要阐明维生素 D 的活性代谢物在银屑病等病理皮肤状况下所产生的潜在影响。在紫外线 B (UVB) 照射下，皮肤可以自主合成、激活和降解维生素 D。在这项初步案例研究中，我们首次在一名银屑病患者的皮肤活检标本中使用飞行时间二次离子质谱 (ToF-SIMS) 进行分析，这些标本在接受 UVB 光疗前后均进行了分析。我们能够在皮肤和皮下组织中可视化维生素 D 及其代谢物。同时，我们还获得了关于它们在亚细胞水平的定位以及皮肤形态的信息。这项研究证明，ToF-SIMS 是一种很有前途的强大工具，可用于通过皮肤活检研究维生素 D 在皮肤科疾病中的作用。
