Santos Vitor Hugo Dos, Pfeifer João Pedro Hübbe, Souza Jaqueline Brandão De, Stievani Fernanda De Castro, Hussni Carlos Alberto, Golim Marjorie De Assis, Deffune Elenice, Alves Ana Liz Garcia
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
School of Medicine, Blood Centre Division, São Paulo State University (UNESP), Botucatu, SP, Brazil.
Res Vet Sci. 2019 Jun;124:38-45. doi: 10.1016/j.rvsc.2019.02.005. Epub 2019 Feb 25.
Osteoarthritis is an incapacitating disease characterized by pain and a progressive decrease in joint mobility. The implantation of mesenchymal stem cells (MSCs) has shown promising results for its treatment. The challenge remains to keep the cells longer at the site of action, increasing their therapeutic potential. The aim of this study was to evaluate the effectiveness of the Qtracker® 655 nanocrystal marking on allogeneic synovial membrane (SM) MSCs, encapsulated in alginate hydrogel, evaluating the migration of these cells. The 10 radiocarpal joints were submitted to arthroscopic surgery (D0), divided into two groups. The chondral defect was treated according to the group: GA free-labelled MSC and GB labelled MSC microcapsules. Seven days after lesion induction and implantation of labelled cells, biopsies of the lesion site were performed in two animals, and fragments of SM and joint capsule also collected, which were frozen and later processed for fluorescence microscopy. The synovial fluid of the three animals was analyzed by flow cytometry three times - 3, 7 and 21 days after application. The cellular marking with the nanocrystals allowed the visualization of the cells in cartilage, synovial membrane, synovial fluid and articular capsule, but with a predilection for the synovial membrane and the lesion site was scarce. The labelled MSC in microcapsules were scarce in the synovial fluid and could be related to the small quantity of MSCs leaving the pores of the microcapsules, also favorable results, as the cells release paracrine effects acting for a long period until the cellular differentiation.
骨关节炎是一种使人丧失能力的疾病，其特征为疼痛和关节活动度逐渐降低。间充质干细胞（MSCs）植入已显示出对其治疗有前景的结果。挑战仍然是使细胞在作用部位停留更长时间，增加其治疗潜力。本研究的目的是评估包裹在藻酸盐水凝胶中的同种异体滑膜（SM）MSCs上Qtracker® 655纳米晶体标记的有效性，评估这些细胞的迁移情况。10个桡腕关节接受关节镜手术（D0），分为两组。根据分组治疗软骨缺损：GA组为未标记的MSCs，GB组为标记的MSCs微胶囊。在损伤诱导和标记细胞植入7天后，对两只动物的损伤部位进行活检，并收集滑膜和关节囊碎片，将其冷冻，随后进行荧光显微镜处理。在应用后3、7和21天对三只动物的滑液进行三次流式细胞术分析。纳米晶体细胞标记使得能够在软骨、滑膜、滑液和关节囊中观察到细胞，但滑膜和损伤部位的细胞标记较少。微胶囊中的标记MSCs在滑液中较少，这可能与离开微胶囊孔隙的MSCs数量少有关，这也是有利结果，因为细胞释放的旁分泌效应可长期起作用直至细胞分化。
