Kawcak C E, Frisbie D D, Trotter G W, McIlwraith C W, Gillette S M, Powers B E, Walton R M
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
Am J Vet Res. 1997 Oct;58(10):1132-40.
To evaluate the effects of arthroscopic surgery, osteochondral fragmentation, and treatment with IV administered hyaluronate on histologic, histochemical, and biochemical measurements within the carpal joints of horses.
12 clinically normal horses, 2 to 7 years of age.
Horses had an osteochondral fragment created at the distal aspect of the radiocarpal bone of 1 randomly chosen middle carpal joint to simulate osteochondral fragmentation. Horses were treated with 40 mg of hyaluronate or saline solution (placebo) intravenously once a week for 3 consecutive weeks (days 13, 20, and 27 after surgery). Treadmill exercise proceeded 5 days per week beginning 15 days, and ending 72 days, after surgery. Clinical evaluations were performed at the beginning and end of the study. Synovial fluid samples were obtained aseptically from both middle carpal joints on days 0, 13, 20, 27, 34, and 72 after surgery, and total protein, inflammatory cell, hyaluronate, glycosaminoglycan, and prostaglandin E2 concentrations were measured in each sample. All horses were euthanatized on day 72. Synovial membrane and articular cartilage were obtained for histologic evaluation. Articular cartilage samples were also obtained aseptically for determining glycosaminoglycan content and chondrocyte synthetic rate for glycosaminoglycans.
Horses treated with hyaluronate intravenously had lower lameness scores (were less lame), significantly better synovial membrane histologic scores, and significantly lower concentrations of total protein and prostaglandin E2 within synovial fluid 72 days after surgery, compared with placebo-treated horses. Treatment with intravenously administered hyaluronate had no significant effects on glycosaminoglycan content, synthetic rate or morphologic scoring in articular cartilage, or other synovial fluid measurements.
Intravenously administered hyaluronate appears to alleviate signs of lameness by interacting with synoviocytes, and by decreasing production and release of inflammatory mediators.
评估关节镜手术、骨软骨碎片形成以及静脉注射透明质酸盐治疗对马腕关节内组织学、组织化学和生物化学指标的影响。
12匹2至7岁临床健康的马。
在1个随机选择的中腕关节的桡腕骨远端制造1个骨软骨碎片，以模拟骨软骨碎片形成。马每周静脉注射1次40mg透明质酸盐或生理盐水（安慰剂），连续注射3周（术后第13、20和27天）。术后15天开始每周进行5天跑步机运动，至术后72天结束。在研究开始和结束时进行临床评估。在术后第0、13、20、27、34和72天，从两个中腕关节无菌采集滑液样本，测定每个样本中的总蛋白、炎症细胞、透明质酸盐、糖胺聚糖和前列腺素E2浓度。所有马均在第72天实施安乐死。获取滑膜和关节软骨进行组织学评估。还无菌采集关节软骨样本，以测定糖胺聚糖含量和软骨细胞合成糖胺聚糖的速率。
与接受安慰剂治疗的马相比，静脉注射透明质酸盐治疗的马跛行评分更低（跛行程度更轻），滑膜组织学评分显著更好，且术后72天时滑液中总蛋白和前列腺素E2浓度显著更低。静脉注射透明质酸盐治疗对关节软骨中的糖胺聚糖含量、合成速率或形态学评分，或其他滑液指标均无显著影响。
静脉注射透明质酸盐似乎通过与滑膜细胞相互作用，并减少炎症介质的产生和释放来减轻跛行症状。
