Seki T, Kikuyama S
Gen Comp Endocrinol. 1986 Feb;61(2):197-202. doi: 10.1016/0016-6480(86)90197-8.
Pituitary glands of bullfrogs (Rana catesbeiana) were incubated in medium containing thyrotropin-releasing hormone (TRH) and/or dopamine in order to see their effects on the release and synthesis of prolactin, which was measured by a homologous radioimmunoassay. Prolactin synthesis was measured by monitoring the incorporation of [3H]leucine into prolactin. TRH (0.1-10 ng/ml) stimulated the release of immunoassayable prolactin and newly synthesized [3H]prolactin into the medium in a dose-dependent manner; however, it was ineffective in increasing total prolactin (medium plus pituitary) and the incorporation of [3H]leucine into the total prolactin during the experimental period (20 hr). The TRH-induced elevation of prolactin release was suppressed by the addition of dopamine (5 X 10(-7) M) to the medium.
为了观察促甲状腺激素释放激素（TRH）和/或多巴胺对牛蛙（牛蛙属）垂体催乳素释放和合成的影响，将牛蛙垂体置于含有TRH和/或多巴胺的培养基中培养，催乳素通过同源放射免疫测定法进行测量。催乳素的合成通过监测[3H]亮氨酸掺入催乳素来测定。TRH（0.1 - 10 ng/ml）以剂量依赖的方式刺激可免疫测定的催乳素和新合成的[3H]催乳素释放到培养基中；然而，在实验期间（20小时），它对增加总催乳素（培养基加垂体）以及[3H]亮氨酸掺入总催乳素无效。向培养基中添加多巴胺（5×10(-7) M）可抑制TRH诱导的催乳素释放升高。
