Twining S S, Lohr K M, Moulder J E
Invest Ophthalmol Vis Sci. 1986 Apr;27(4):507-15.
A model to study the immune system in Pseudomonas keratitis was developed using defined flora rats (WAG/RijMCW) that have not been exposed to Pseudomonas aeruginosa. One group of rats was made immunocompetent towards P. aeruginosa by intraperitoneal injection of phenol-killed P. aeruginosa while a second group remained naive to this organism. Corneas of both groups were scratched centrally with a 21-g needle, before inoculation with 2 X 10(8) P. aeruginosa organisms. Corneas of control animals were either only scratched or only inoculated with the bacterium. At 18 hr, the naive animals were killed. In naive rat corneas, light and electron microscopy showed bacteria throughout the cornea, polymorphonuclear leukocytes (PMNs) distributed from the limbus towards the center, and little stromal degradation. In contrast, massive corneal degradation was observed in the immunocompetent rats; PMNs were present, but no bacteria were observed free in the stroma. The total acid protease content was higher in the immunocompetent than in the naive rat corneas, a possible reason for the observed difference in corneal degradation. This difference was not due to increased numbers of PMNs since nearly equal numbers of PMNs were counted after enzymatic disaggregation of both types of corneas. Glycogen-induced peritoneal PMNs from both types of rats migrated equally well towards P. aeruginosa culture media and media of corneas incubated with this bacterium. The authors conclude that immune recognition is (1) involved in the corneal host response to P. aeruginosa and (2) required for efficient phagocytosis by PMNs but not their recruitment.
利用未接触过铜绿假单胞菌的特定菌群大鼠（WAG/RijMCW）建立了一种研究角膜假单胞菌感染中免疫系统的模型。一组大鼠通过腹腔注射经苯酚灭活的铜绿假单胞菌使其对该菌具有免疫活性，而另一组大鼠对该菌仍无免疫。两组大鼠的角膜均用21号针头在中央划痕，然后接种2×10⁸个铜绿假单胞菌。对照动物的角膜要么只划痕，要么只接种该细菌。18小时后，处死未免疫的动物。在未免疫大鼠的角膜中，光学显微镜和电子显微镜检查显示整个角膜均有细菌，多形核白细胞（PMN）从角膜缘向中央分布，且基质降解较少。相比之下，在免疫活性大鼠中观察到大量角膜降解；存在PMN，但在基质中未观察到游离细菌。免疫活性大鼠角膜中的总酸性蛋白酶含量高于未免疫大鼠角膜，这可能是观察到的角膜降解差异的原因。这种差异并非由于PMN数量增加，因为对两种类型的角膜进行酶解后计数的PMN数量几乎相等。来自两种类型大鼠的糖原诱导腹腔PMN向铜绿假单胞菌培养基以及与该细菌共同孵育的角膜培养基的迁移能力相同。作者得出结论，免疫识别（1）参与角膜对铜绿假单胞菌的宿主反应，（2）是PMN有效吞噬所必需的，但不是其募集所必需的。
