Sakagami H, Takeda K, Makino Y, Konno K
Jpn J Cancer Res. 1986 Jan;77(1):59-64.
The effects of hot-water extract of pine cone (PCE) of Pinus parviflora Sieb. et Zucc. on the growth and differentiation of ML-1 cells, derived from a patient with human myeloblastic leukemia, were investigated. Growth of ML-1 cells was slightly inhibited at 3% (v/v) PCE, and a cytotoxic effect appeared at greater than 10%. Growth inhibition was accompanied by conversion to morphologically macrophage-like cells with alpha-naphthyl acetate esterase activity. In contrast, PCE dose-dependently increased the Fc receptor and nitroblue tetrazolium (NBT)-reducing activity up to 3%; above 3% its effect declined. Most of the cytotoxic activity was extracted from PCE with ethanol, and separated from the insoluble pellet, which contained the differentiation-inducing activity. The differentiation-inducing activity was eluted near the void volume on Sephadex G-200 gel filtration, with a 260-fold increase in the specific activity.
研究了日本五针松球果热水提取物（PCE）对源自一名人类髓细胞白血病患者的ML-1细胞生长和分化的影响。在3%（v/v）PCE时，ML-1细胞的生长略有抑制，而在大于10%时出现细胞毒性作用。生长抑制伴随着细胞转化为具有α-萘乙酸酯酶活性的形态学上类似巨噬细胞的细胞。相比之下，PCE在高达3%时剂量依赖性地增加Fc受体和硝基蓝四氮唑（NBT）还原活性；高于3%时其作用下降。大部分细胞毒性活性用乙醇从PCE中提取出来，并与含有分化诱导活性的不溶性沉淀分离。分化诱导活性在Sephadex G-200凝胶过滤中在近空体积处洗脱，比活性增加了260倍。
