School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, Helongjiang, China.
Molecules. 2013 Aug 19;18(8):9933-48. doi: 10.3390/molecules18089933.
Pinus koraiensis polysaccharides (PKP) were extracted by hot water from P. koraiensis pine cones. Five polysaccharide fractions named PKP-A, PKP-B, PKP-C, PKP-D and PKP-E were successfully separated at final ethanol concentrations of 30%, 50%, 60%, 70% and 80%, respectively. HPLC, FT-IR, GC-MS and automatic amino-acid analysis were applied to investigate their chemical characteristics. Monosaccharide component analysis indicated that the five fractions were all composed of D-ribose, L-rhamnose, L-arabinose, D-xylose, D-mannose, D-glucose and D-galactose, but their molar ratios were quite different. HPLC results revealed that the polysaccharides precipitated by higher concentrations of ethanol solution had lower molecular masses. Moreover, the antioxidant activities of the five fractions were studied on the basis of hydroxyl radical and ABTS radical scavenging tests. The five graded polysaccharide fractions exhibited good inhibitory power, and MTT tests in vitro showed the IC50 of PKP-A and PKP-E were 1,072.5 and 2,070.0 μg · mL-1, respectively. These results demonstrated that the PKP could be a potential source of natural antioxidants or dietary supplements.
红松松塔多糖（PKP）经热水提取，最后用 30%、50%、60%、70%和 80%的乙醇溶液沉淀，成功分离出 5 种多糖组分，分别命名为 PKP-A、PKP-B、PKP-C、PKP-D 和 PKP-E。采用高效液相色谱（HPLC）、傅里叶变换红外光谱（FT-IR）、气相色谱-质谱联用（GC-MS）和自动氨基酸分析等方法对其化学性质进行了研究。单糖组成分析表明，这 5 种多糖均由 D-核糖、L-鼠李糖、L-阿拉伯糖、D-木糖、D-甘露糖、D-葡萄糖和 D-半乳糖组成，但摩尔比差异较大。HPLC 结果表明，高浓度乙醇溶液沉淀的多糖分子量较低。此外，还基于羟基自由基和 ABTS 自由基清除试验研究了 5 种分级多糖的抗氧化活性。这 5 种分级多糖均具有良好的抑制能力，MTT 体外试验表明 PKP-A 和 PKP-E 的 IC50 分别为 1072.5 和 2070.0 μg·mL-1。这些结果表明，红松松塔多糖可能是天然抗氧化剂或膳食补充剂的潜在来源。
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