非编码RNA介导能量心理学的疗效吗？
Do Noncoding RNAs Mediate the Efficacy of Energy Psychology?
作者信息
Yount Garret, Church Dawson, Rachlin Kenneth, Blickheuser Katharina, Cardonna Ippolito
机构信息
Institute of Noetic Sciences, Petaluma, California.
National Institute for Integrative Healthcare, Fulton, California.
出版信息
Glob Adv Health Med. 2019 Feb 25;8:2164956119832500. doi: 10.1177/2164956119832500. eCollection 2019.
BACKGROUND
There are over 100 published studies of a therapy called Emotional Freedom Techniques (EFT). This popular form of energy psychology combines elements of established methods like cognitive therapy with acupressure. Our group reported the first evidence of its mechanisms of action at the molecular level, showing that it can influence levels of the stress hormone cortisol.
OBJECTIVES
Given recent advances in molecular genomics that have identified noncoding ribonucleic acid (RNA) molecules as important regulators of gene expression, the aim of this study is to explore the possibility that microRNAs play a role in mediating the effects of EFT.
METHODS
We measured microRNA levels in stored blood samples from our previous study in which veterans were randomized into an EFT group receiving EFT and treatment as usual throughout a 10-week intervention period, and a control group receiving only treatment as usual during the intervention period and then receiving EFT. A broad panel of 800 microRNAs was probed using a multiplexed, direct hybridization, and detection system.
RESULTS
All of the microRNA targets were expressed at low levels and most were below thresholds established by negative control probes. Baseline variability was determined using samples collected from the control group at the start and end of the intervention period, and used to filter out targets that were too noisy under control conditions to be able to distinguish a response to treatment. Analysis of the remaining viable targets found a general trend of reduced expression following EFT, compared to expression levels in samples from the control group during the intervention period. The most notable decreases in expression levels were found for 2 microRNAs: let-7b and let-7c, although no significance was found after adjusting for multiple comparisons.
CONCLUSIONS
These preliminary data support the feasibility of measuring microRNA expression level changes that correlate with effective EFT therapy.
背景
有超过100项已发表的关于一种名为情绪释放技术（EFT）的疗法的研究。这种流行的能量心理学形式将认知疗法等既定方法的元素与指压疗法相结合。我们的团队报告了其在分子水平上作用机制的首个证据，表明它可以影响应激激素皮质醇的水平。
目的
鉴于分子基因组学的最新进展已将非编码核糖核酸（RNA）分子确定为基因表达的重要调节因子，本研究的目的是探讨微小RNA在介导EFT作用中发挥作用的可能性。
方法
我们在之前研究的储存血液样本中测量了微小RNA水平。在该研究中，退伍军人被随机分为EFT组和对照组。EFT组在为期10周的干预期内接受EFT和常规治疗，对照组在干预期内仅接受常规治疗，然后接受EFT。使用多重直接杂交和检测系统对800种微小RNA的广泛面板进行检测。
结果
所有微小RNA靶标的表达水平都很低，大多数低于阴性对照探针设定的阈值。使用干预期开始和结束时从对照组收集的样本确定基线变异性，并用于筛选在对照条件下噪声过大而无法区分治疗反应的靶标。对其余可行靶标的分析发现，与干预期内对照组样本的表达水平相比，EFT后表达水平总体呈下降趋势。发现2种微小RNA（let-7b和let-7c）的表达水平下降最为显著，尽管在进行多重比较校正后未发现显著性差异。
结论
这些初步数据支持测量与有效EFT治疗相关的微小RNA表达水平变化的可行性。
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