Matic Toni, Bojanic Ines, Mazic Sanja, Golubic Cepulic Branka, Bilic Ernest
Department of Pediatrics, University Hospital Center Zagreb, Zagreb, Croatia.
Department of Transfusion Medicine and Transplantation Biology, University Hospital Center Zagreb, Zagreb, Croatia.
J Clin Apher. 2019 Aug;34(4):468-473. doi: 10.1002/jca.21700. Epub 2019 Mar 4.
A mini extracorporeal photopheresis (mini-ECP) "off line" technique has been developed for use in the treatment of small children and patients with apheresis contraindications. Until now various methods have been used for buffy coat separation from whole blood. In this report we describe a protocol for mini buffy coat preparation using the automated Sepax laboratory separator for "off line" ECP treatment in a low body weight child with graft-vs-host-disease. According to our results this alternative method has been proven feasible and tolerable.
一种用于治疗小儿及有单采禁忌证患者的“离线”小型体外光分离置换术（mini - ECP）技术已被研发出来。到目前为止，已采用多种方法从全血中分离出 Buffy 层。在本报告中，我们描述了一种使用自动 Sepax 实验室分离器制备小型 Buffy 层的方案，用于对一名患有移植物抗宿主病的低体重儿童进行“离线”ECP 治疗。根据我们的结果，这种替代方法已被证明是可行且可耐受的。
