Grabmer Christoph, Schlager Sandra, Mayer Georg, Streif Doris, Lener Thomas, Schallmoser Katharina, Rohde Eva
Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.
Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria.
J Clin Apher. 2017 Feb;32(1):12-15. doi: 10.1002/jca.21455. Epub 2016 Mar 4.
Extracorporeal photopheresis (ECP) is an important cell-based therapy for various diseases but is limited to patients eligible for apheresis. We developed an alternative mini buffy coat (BC) preparation method using the Spectra Optia® apheresis system and compared its efficacy of white blood cell (WBC) recovery with the standard mini BC preparation method already established for pediatric patients.
Whole blood (450 ± 45 mL) samples were collected from 30 randomly selected healthy volunteer blood donors and divided into two groups. In the first group, WBCs were separated with a fully automated separator device (Compomat G4 ). In the second group, BCs were separated with the bone marrow processing program of the Spectra Optia apheresis system.
There were no significant differences in total leukocyte counts per product between the two groups. In contrast, lymphocyte counts per product were significantly higher (P < 0.001) in BCs separated from apheresis.
Our novel technique resulted in similar WBC yields but higher lymphocyte yields than the standard mini BC preparation method. This method can serve as an alternative to WBC collection in conventional ECP for adult patients with apheresis contraindications. J. Clin. Apheresis 32:12-15, 2017. © 2016 Wiley Periodicals, Inc.
体外光化学疗法（ECP）是一种针对多种疾病的重要细胞疗法，但仅限于适合进行血液成分单采的患者。我们开发了一种使用Spectra Optia®血液成分单采系统的替代性小量血沉棕黄层（BC）制备方法，并将其白细胞（WBC）回收效果与已为儿科患者确立的标准小量BC制备方法进行了比较。
从30名随机选择的健康志愿献血者采集全血（450±45 mL）样本，并分为两组。第一组使用全自动分离设备（Compomat G4）分离白细胞。第二组使用Spectra Optia血液成分单采系统的骨髓处理程序分离血沉棕黄层。
两组之间每个产品的总白细胞计数无显著差异。相比之下，通过血液成分单采分离的血沉棕黄层中每个产品的淋巴细胞计数显著更高（P<0.001）。
我们的新技术产生的白细胞产量与标准小量BC制备方法相似，但淋巴细胞产量更高。对于有血液成分单采禁忌证的成年患者，这种方法可作为传统ECP中白细胞采集的替代方法。《临床血液成分单采杂志》32:12 - 15, 2017。©2016威利期刊公司
