Biotechnology of Natural Products, Technische Universität München, Liesel-Beckmann-Str. 1, 85354, Freising, Germany.
Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee, Scotland, DD2 5DA, UK.
Metabolomics. 2018 Oct 26;14(11):145. doi: 10.1007/s11306-018-1441-x.
The qualitative and quantitative analysis of all low molecular weight metabolites within a biological sample, known as the metabolome, provides powerful insights into their roles in biological systems and processes. The study of all the chemical structures, concentrations, and interactions of the thousands of metabolites is called metabolomics. However present state of the art methods and equipment can only analyse a small portion of the numerous, structurally diverse groups of chemical substances found in biological samples, especially with respect to samples of plant origin with their huge diversity of secondary metabolites. Nevertheless, metabolite profiling and fingerprinting techniques have been applied to the analysis of the strawberry metabolome since their early beginnings.
The application of metabolomics and metabolite profiling approaches within strawberry research was last reviewed in 2011. Here, we aim to summarize the latest results from research of the strawberry metabolome since its last review with a special emphasis on studies that address specific biological questions.
Analysis of strawberry, and other fruits, requires a plethora of analytical methods and approaches encompassing the analysis of primary and secondary metabolites, as well as capturing and quantifying volatile compounds that are related to aroma as well as fruit development, function and plant-to-plant communication. The success and longevity of metabolite and volatile profiling approaches in fruit breeding relies upon the ability of the approach to uncover biologically meaningful insights. The key concepts that must be addressed and are reviewed include: gene function analysis and genotype comparison, analysis of environmental effects and plant protection, screening for bioactive compounds for food and non-food uses, fruit development and physiology as well as fruit sensorial quality. In future, the results will facilitate fruit breeding due to the identification of metabolic QTLs and candidate genes for fruit quality and consumer preference.
对生物样本中所有低分子量代谢物进行定性和定量分析，即代谢组学，可以深入了解它们在生物系统和过程中的作用。对数千种代谢物的化学结构、浓度和相互作用的研究称为代谢组学。然而，目前的技术和设备只能分析生物样本中发现的大量结构多样的化学物质的一小部分，尤其是对于植物来源的样本，其具有巨大的次生代谢物多样性。尽管如此，代谢物分析和指纹图谱技术已应用于草莓代谢组的分析。
代谢组学和代谢物分析方法在草莓研究中的应用在 2011 年进行了综述。在这里，我们旨在总结自上次综述以来草莓代谢组研究的最新结果，特别强调解决特定生物学问题的研究。
草莓和其他水果的分析需要大量的分析方法和途径，包括对初级和次级代谢物的分析，以及捕获和定量与香气以及水果发育、功能和植物间通讯相关的挥发性化合物。代谢物和挥发性分析方法在水果育种中的成功和长期应用依赖于该方法揭示具有生物学意义的见解的能力。必须解决并进行综述的关键概念包括：基因功能分析和基因型比较、环境效应和植物保护分析、生物活性化合物的筛选用于食品和非食品用途、水果发育和生理学以及水果感官质量。未来，由于鉴定与果实品质和消费者偏好相关的代谢 QTL 和候选基因，这些结果将有助于水果的选育。
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