Pont Simon D A, Allwood J William
Environmental and Biochemical Sciences, James Hutton Institute, Dundee, Scotland, UK.
Methods Mol Biol. 2025;2895:227-243. doi: 10.1007/978-1-0716-4350-1_15.
Profiling of secondary metabolites within Fragaria sp. (strawberry), Rubus sp. (raspberries and blackberries), and Ribes sp. (blackcurrants and redcurrants) is of key importance with respect to the nutritional (antioxidant) benefits associated with a range of polyphenol classes within such fruits and how they vary between growth environment and season, as well as their deployment as quantitative trait loci (QTL) within breeding populations. This chapter presents an ultra-high-performance liquid chromatography (UHPLC) approach applying aqueous functionalized C18 chromatography combined with mass spectrometry (MS) for the characterization and relative quantification of a wide range of polyphenols such as the pelargonidins, being rich in strawberry; cyanidins, procyanidins, and anthocyanins, being rich in raspberry; and delphinidins and prodelphinidins, being particularly rich in blackcurrant, as well as a range of colorless flavonoids, and amino and organic acids.
对草莓属（草莓）、悬钩子属（树莓和黑莓）以及醋栗属（黑加仑和红加仑）植物中的次生代谢产物进行分析，对于这些水果中一系列多酚类物质所带来的营养（抗氧化）益处、它们在不同生长环境和季节中的变化情况，以及它们作为数量性状基因座（QTL）在育种群体中的应用而言至关重要。本章介绍了一种超高效液相色谱（UHPLC）方法，该方法采用水性官能化C18色谱与质谱（MS）联用，用于对多种多酚进行表征和相对定量，例如富含于草莓中的天竺葵色素；富含于树莓中的矢车菊素、原花青素和花青素；特别富含于黑加仑中的飞燕草素和原飞燕草素，以及一系列无色黄酮类化合物、氨基酸和有机酸。
