Department of Operative Dentistry, Faculdade de Medicina Dentária da Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393, Porto, Portugal.
Department of Dental Biomaterials, Faculdade de Medicina Dentária da Universidade de Lisboa, Rua Professora Teresa Ambrósio, Cidade Universitária, 1600-277, Lisbon, Portugal.
Lasers Med Sci. 2019 Sep;34(7):1493-1501. doi: 10.1007/s10103-019-02749-w. Epub 2019 Mar 4.
The proanthocyanidin (PA)-rich grape seed extract (GSE) is a collagen cross-linking agent that can perform a chemical bond with the dentin's collagen. The objective of this study was to evaluate the influence on shear bond strength (SBS) of the pre-conditioning of GSE, on human dentin surfaces conditioned with Er,Cr:YSGG laser. The sample consisted of 64 non-carious human teeth, divided into eight groups, four groups conditioned with Er,Cr:YSGG laser (4.5 W, 50 Hz, 50 μs, 70% air, 90% water) and four prepared with conventional methods (control). In both groups, a GSE solution was applied before using the two adhesives tested: Clearfil SE Bond (CSE) and Scotchbond Universal (SU). Subsequently, a SBS test, a scanning electron microscopy, and a statistical analysis were performed. In the laser groups, the best SBS mean (20.08 ± 4.01 MPa) was achieved in the group treated with GSE and CSE. The control group with the application of CSE showed the highest SBS mean (24.27 ± 10.28 MPa), and the group treated with laser and SU showed the lowest SBS mean (12.94 ± 6.51 MPa). Between these two groups there was a statistically significant difference (p = 0.05). However, this was not observed among the laser or control groups. The type of dentin surface preparation can influence the SBS. The CSE showed better SBS in laser and control groups. The presence of GSE did not improve the adhesion on surfaces conditioned with laser, but more studies should be carried out in the future to confirm this conclusion.
富含原花青素（PA）的葡萄籽提取物（GSE）是一种能够与牙本质胶原形成化学键的胶原交联剂。本研究旨在评估 GSE 预处理对 Er,Cr:YSGG 激光处理后的人牙本质表面剪切粘结强度（SBS）的影响。样本由 64 颗无龋人牙组成，分为 8 组，4 组用 Er,Cr:YSGG 激光（4.5 W，50 Hz，50 μs，70%空气，90%水）处理，4 组用传统方法处理（对照）。在这两组中，在使用两种测试的胶粘剂（Clearfil SE Bond [CSE] 和 Scotchbond Universal [SU]）之前，均应用 GSE 溶液。随后进行 SBS 测试、扫描电子显微镜检查和统计分析。在激光组中，用 GSE 和 CSE 处理的组的 SBS 平均值（20.08 ± 4.01 MPa）最高。应用 CSE 的对照组的 SBS 平均值（24.27 ± 10.28 MPa）最高，而用激光和 SU 处理的组的 SBS 平均值最低（12.94 ± 6.51 MPa）。两组之间存在统计学差异（p=0.05）。然而，在激光组或对照组中均未观察到这种差异。牙本质表面处理类型会影响 SBS。CSE 在激光组和对照组中表现出更好的 SBS。GSE 的存在并没有改善激光处理后的表面粘结，但是未来应该进行更多的研究来证实这一结论。
