Gill J C, Menitove J E, Anderson P R, Casper J T, Devare S G, Wood C, Adair S, Casey J, Scheffel C, Montgomery R R
J Pediatr. 1986 Apr;108(4):511-6. doi: 10.1016/s0022-3476(86)80824-1.
We investigated the relationship of the presence of antibodies to HTLV-III and immunologic abnormalities in patients with hemophilia. Serum antibodies to HTLV-III were analyzed by ELISA assay, immunoprecipitation of labeled cell extracts, and immunoprecipitation of purified HTLV-III p24. Thirty-four (61%) of the total group (n = 56) had antibody to HTLV-III; 34 (76%) of 45 patients given commercial factor VIII preparations were seropositive, compared with none of 11 patients treated exclusively with cryoprecipitate obtained from volunteer blood donors. Of patients who were seropositive for HTLV-III antibody, 94% had abnormal T4/T8 ratios, and 33% of those whose serum was antibody negative had abnormal T4/T8 ratios; five patients, each antibody positive, have lymphadenopathy syndrome. Sequential studies in a subset of patients indicate that there is a changing pattern of antibody production to HTLV-III antigens after seroconversion.
我们研究了血友病患者中抗人类嗜T淋巴细胞病毒III型（HTLV-III）抗体的存在与免疫异常之间的关系。通过酶联免疫吸附测定（ELISA）、标记细胞提取物的免疫沉淀以及纯化的HTLV-III p24的免疫沉淀来分析血清中抗HTLV-III抗体。在整个研究组（n = 56）中，34例（61%）有抗HTLV-III抗体；在接受商业性因子VIII制剂治疗的45例患者中，34例（76%）血清学呈阳性，而在仅接受从志愿献血者获得的冷沉淀物治疗的11例患者中无一例呈阳性。在抗HTLV-III抗体血清学呈阳性的患者中，94%的患者T4/T8比值异常，而在血清抗体阴性的患者中，33%的患者T4/T8比值异常；5例抗体均为阳性的患者患有淋巴结病综合征。对一部分患者的连续研究表明，血清转化后针对HTLV-III抗原的抗体产生模式在发生变化。
