Hei S M, Wei H J, Chen H, Wang J G
Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao 266071, China.
Department of Pathology, Qingdao Municipal Hospital, Qingdao 266071, China.
Zhonghua Bing Li Xue Za Zhi. 2019 Mar 8;48(3):225-230. doi: 10.3760/cma.j.issn.0529-5807.2019.03.011.
To detect the expression of New York esophageal squamous cell carcinoma antigen 1 (NY-ESO-1) in common types of mesenchymal myxoid tumors, and to investigate its significance in the diagnosis and differential diagnosis of myxoid liposarcoma. A total of 43 formalin-fixed paraffin-embedded samples of mesenchymal myxoid tumors from the Affiliated Hospital of Qingdao University and Qingdao Municipal Hospital ranging between 2010 and 2017 were selected. NY-ESO-1 expression was detected by immunohistochemical staining. DDIT3 gene status was detected by fluorescence in situ hybridization (FISH). NY-ESO-1 mRNA was detected by reverse transcription-PCR (RT-PCR). Histopathology and FISH results confirmed that there were 11 cases of myxoid liposarcoma and 32 other types (including 7 cases of well-differentiated liposarcoma, 1 dedifferentiated liposarcoma, 3 lipomas, 2 lipoblastomas and 19 non-adipocytic tumors). Immunohistochemical staining showed that the positive expression propotion of NY-ESO-1 in myxoid liposarcoma was 11/11, and the positive location was the cytoplasm and nucleus of lipoblast cells. The expression intensity is higher in regions with round cell differentiation. Among the 32 cases of other mesenchymal myxoid tumors, only one well-differentiated liposarcoma showed positive immunoreactivity for NY-ESO-1. RT-PCR confirmed that 7 cases of myxoid liposarcoma (7/11) and one well-differentiated liposarcoma (1/7) had NY-ESO-1 mRNA expression. NY-ESO-1 is positively expressed in myxoid liposarcoma. It can be served as a useful marker for the diagnosis and differential diagnosis of myxoid liposarcoma.
检测纽约食管鳞状细胞癌抗原1（NY-ESO-1）在常见类型间叶性黏液样肿瘤中的表达情况，并探讨其在黏液样脂肪肉瘤诊断及鉴别诊断中的意义。选取2010年至2017年青岛大学附属医院和青岛市市立医院共43例经福尔马林固定、石蜡包埋的间叶性黏液样肿瘤样本。采用免疫组织化学染色检测NY-ESO-1表达，荧光原位杂交（FISH）检测DDIT3基因状态，逆转录-聚合酶链反应（RT-PCR）检测NY-ESO-1 mRNA。组织病理学及FISH结果证实，黏液样脂肪肉瘤11例，其他类型32例（包括高分化脂肪肉瘤7例、去分化脂肪肉瘤1例、脂肪瘤3例、脂肪母细胞瘤2例及非脂肪细胞性肿瘤19例）。免疫组织化学染色显示，黏液样脂肪肉瘤中NY-ESO-1阳性表达率为11/11，阳性定位在脂肪母细胞的细胞质和细胞核，在圆形细胞分化区域表达强度较高。在32例其他间叶性黏液样肿瘤中，仅1例高分化脂肪肉瘤NY-ESO-1免疫反应阳性。RT-PCR证实，7例黏液样脂肪肉瘤（7/11）及1例高分化脂肪肉瘤（1/7）有NY-ESO-1 mRNA表达。NY-ESO-1在黏液样脂肪肉瘤中呈阳性表达，可作为黏液样脂肪肉瘤诊断及鉴别诊断的有用标志物。
