Chan J Y, Walfish P G
Mol Biol Med. 1986 Feb;3(1):99-112.
Lymphocytes from patients with Graves' disease and Hashimoto's thyroiditis were analysed directly by a dual laser-activated cell-sorter for the percentage of activated T-cells and their subsets using two-colour dye-labelling with monoclonal antibodies specific for antigens of Ia+ T-cells, as well as either their T-helper/inducer (TH/I) or T-suppressor/cytotoxic (TS/C) subsets. Compared to normal subjects, patients with hyperthyroid Graves' disease had a significantly (p less than 0.0001) higher percentage of activated (Ia+ Leu 4+) T-cells (7.8 +/- 1.9 versus 1.9 +/- 0.4), wherein their percentage of Ia+ Leu 3a+ (Ia+ TH/I) subset was significantly increased (p less than 0.001) and the percentage of Ia+ Leu 2a+ (Ia+ TS/C) subset decreased; patients with hypothyroid Hashimoto's thyroiditis also had a significant (p less than 0.001) increase in the percentage of Ia+ T-cells (4.3 +/- 0.6 versus 1.9 +/- 0.4), wherein their percentage of Ia+ TH/I was significantly reduced (p less than 0.001), while the percentage of the Ia+ TS/C subset was increased compared to normal. Following a return of thyroid status to euthyroidism, the percentage of Ia+ T-cells decreased in both Graves' and Hashimoto's thyroiditis patients, but remained significantly higher (p less than 0.001) than normal subjects, while the Ia+ TH/I and Ia+ TS/C subsets were no longer different.
(1) the feasibility of enumerating Ia+ T-cells and their subsets in autoimmune thyroid diseases using two-coloured dyes and dual laser flow microfluorometric analytical techniques has been demonstrated. (2) Percentage increases in Ia+ T-cells have been demonstrated in both hyperthyroid Graves' and hypothyroid Hashimoto's which, following restoration to euthyroidism, decreased but still remained significantly higher than normal. (3) Patients with hyperthyroid Graves' and hypothyroid Hashimoto's disease were demonstrated to have the opposite phenotypic changes within activated T-cell subsets, thereby indicating that their pathogenic mechanisms are different.
采用双色染料标记法，使用针对Ia + T细胞抗原以及T辅助/诱导（TH/I）或T抑制/细胞毒性（TS/C）亚群的单克隆抗体，通过双激光激活细胞分选仪直接分析格雷夫斯病和桥本甲状腺炎患者的淋巴细胞，以检测活化T细胞及其亚群的百分比。与正常受试者相比，甲状腺功能亢进的格雷夫斯病患者活化（Ia + Leu 4 +）T细胞的百分比显著更高（p <0.0001）（7.8±1.9对1.9±0.4），其中Ia + Leu 3a +（Ia + TH/I）亚群的百分比显著增加（p <0.001），而Ia + Leu 2a +（Ia + TS/C）亚群的百分比降低；甲状腺功能减退的桥本甲状腺炎患者Ia + T细胞的百分比也显著增加（p <0.001）（4.3±0.6对1.9±0.4），其中Ia + TH/I的百分比显著降低（p <0.001），而Ia + TS/C亚群的百分比与正常相比增加。甲状腺状态恢复至甲状腺功能正常后，格雷夫斯病和桥本甲状腺炎患者的Ia + T细胞百分比均下降，但仍显著高于正常受试者（p <0.001），而Ia + TH/I和Ia + TS/C亚群不再有差异。
（1）已证明使用双色染料和双激光流式微量荧光分析技术在自身免疫性甲状腺疾病中计数Ia + T细胞及其亚群的可行性。（2）在甲状腺功能亢进的格雷夫斯病和甲状腺功能减退的桥本甲状腺炎中均已证明Ia + T细胞百分比增加，恢复至甲状腺功能正常后，该百分比下降，但仍显著高于正常。（3）甲状腺功能亢进的格雷夫斯病和甲状腺功能减退的桥本甲状腺炎患者在活化T细胞亚群中表现出相反的表型变化，从而表明它们的致病机制不同。
