Sridama V, Pacini F, DeGroot L J
J Clin Endocrinol Metab. 1982 Feb;54(2):316-9. doi: 10.1210/jcem-54-2-316.
Monoclonal antibodies reacting with cell surface antigens of helper (T4), suppressor (T8) T cells and common T-cell antigen (T3) were used by an immunofluorescence technique to enumerate peripheral T-lymphocytes in 42 patients with Graves' disease and 16 patients with Hashimoto's thyroiditis. The percentages of total T cells (cells which react with anti-T3) and helper/inducer cells (cells which react with anti-T4) among peripheral mononuclear cells in Graves' and Hashimoto's patients were not significantly different from those found in normal controls, except for a decrease in cells which react with anti-T3 in toxic Graves' disease without medication. The most important finding was a decrease in the percentage of cytotoxic/suppressor T cells (cells which react with anti-T8) in toxic Graves' disease and Hashimoto's thyroiditis. In patients with Graves' disease who were hyperthyroid or euthyroid on propylthiouracil treatment and euthyroid after radioactive iodide treatment, the percentage of cells which react with anti-T8 was also decreased, but this did not reach statistical significance. These findings support the hypothesis of defects in suppressor T-lymphocytes in autoimmune thyroid diseases.
采用免疫荧光技术，使用与辅助性（T4）、抑制性（T8）T细胞以及常见T细胞抗原（T3）的细胞表面抗原发生反应的单克隆抗体，对42例格雷夫斯病患者和16例桥本甲状腺炎患者的外周血T淋巴细胞进行计数。格雷夫斯病和桥本甲状腺炎患者外周血单个核细胞中总T细胞（与抗T3反应的细胞）和辅助/诱导性T细胞（与抗T4反应的细胞）的百分比，与正常对照组相比无显著差异，但未用药的毒性弥漫性甲状腺肿患者中与抗T3反应的细胞有所减少。最重要的发现是毒性弥漫性甲状腺肿和桥本甲状腺炎中细胞毒性/抑制性T细胞（与抗T8反应的细胞）百分比降低。在接受丙硫氧嘧啶治疗时甲状腺功能亢进或正常以及放射性碘治疗后甲状腺功能正常的格雷夫斯病患者中，与抗T8反应的细胞百分比也降低，但未达到统计学意义。这些发现支持自身免疫性甲状腺疾病中抑制性T淋巴细胞存在缺陷的假说。
