Mendoza Azucena, Hernández Fernando, Hernández Sanjuana, Ruíz Daniel, de la Vega Octavio Martínez, Mora Gustavo, Acosta Jorge, Simpson June
Department of Genetic Engineering, CINVESTAV, Unidad Irapuato, Apdo. Postal 629, Irapuato, Guanajuato, Mexico.
Institución de Enseñanza e Investigación en Ciencias Agrícolas, Colegio de Posgraduados, Montecillo, Texcoco, Mexico.
Plant Dis. 2001 Mar;85(3):252-255. doi: 10.1094/PDIS.2001.85.3.252.
Phaseolus vulgaris line A193 has been shown to be widely resistant to Colletotrichum lindemuthianum, including race 1472, one of the most virulent races of C. lindemuthianum characterized. Resistance to C. lindemuthianum race 1472 in P. vulgaris line A193 was investigated in segregating F and F populations from a cross between A193 and Flor de Mayo Bajio (a commercial cultivar highly susceptible to C. lindemuthianum). Resistance to 1472 in A193 was determined to be conditioned by a single dominant gene. Inoculation of crosses between A193 and cultivars Michigan Dark Red Kidney and Perry Marrow with race 1472 suggest that resistance in A193 is conditioned by the Co-1 gene. Inoculation of the cross A193 × Perry Marrow with C. lindemuthianum race 2, demonstrated that resistance to race 2 in Perry Marrow is also conditioned by a single dominant gene and is distinct to resistance in A193 or Michigan Dark Red Kidney. Three AFLP markers (ECAG/MACC-1, EACA/MAGA-2, EAGG/MAAC-8) linked in repulsion to the Co-1 locus were identified by screening the A193 × Flor de Mayo F population with 314 random amplified polymorphic DNA, amplified fragment length polymorphism, and restriction fragment length polymorphism markers. The two most closely linked markers should be useful in marker-assisted selection for Co-1.
菜豆品系A193已被证明对菜豆炭疽菌具有广泛抗性，包括1472小种，该小种是已鉴定的菜豆炭疽菌中最具毒性的小种之一。在A193与弗洛尔德梅奥巴希奥（一种对菜豆炭疽菌高度敏感的商业栽培品种）杂交产生的分离F和F群体中，研究了菜豆品系A193对菜豆炭疽菌1472小种的抗性。已确定A193对1472小种的抗性由单个显性基因控制。用1472小种接种A193与密歇根深红肾豆和佩里髓豆品种之间的杂交种表明，A193中的抗性由Co-1基因控制。用菜豆炭疽菌2小种接种A193×佩里髓豆的杂交种，结果表明佩里髓豆对2小种的抗性也由单个显性基因控制，且与A193或密歇根深红肾豆中的抗性不同。通过用314个随机扩增多态性DNA、扩增片段长度多态性和限制性片段长度多态性标记筛选A193×弗洛尔德梅奥F群体，鉴定出了三个与Co-1基因座呈排斥连锁的AFLP标记（ECAG/MACC-1、EACA/MAGA-2、EAGG/MAAC-8）。两个连锁最紧密的标记应有助于Co-1基因的标记辅助选择。
