Phytopathology. 2000 Jul;90(7):758-61. doi: 10.1094/PHYTO.2000.90.7.758.
ABSTRACT New cultivars of the common bean (Phaseolus vulgaris) with durable resistance to anthracnose can be developed by pyramiding major resistance genes using marker-assisted selection. To this end, it is necessary to identify sources of resistance and molecular markers tightly linked to the resistance genes. The objectives of this work were to study the inheritance of resistance to anthracnose in the cultivar TO (carrying the Co-4 gene), to identify random amplified polymorphic DNA (RAPD) markers linked to Co-4, and to introgress this gene in the cultivar Rudá. Populations F(1), F(2), F(2:3), BC(1)s, and BC(1)r from the cross Rudá x TO were inoculated with race 65 of Colletotrichum lindemuthianum, causal agent of bean anthracnose. The phenotypic ratios (resistant/susceptible) were 3:1 in the F(2) population, 1:1 in the BC(1)s, and 1:0 in the BC(1)r, confirming that resistance to anthracnose in the cultivar TO was monogenic and dominant. Six RAPD markers linked to the Co-4 gene were identified, four in the coupling phase: OPY20(830C) (0.0 centimorgan [cM]), OPC08(900C) (9.7 cM), OPI16(850C) (14.3 cM), and OPJ01(1,380C) (18.1 cM); and two in the repulsion phase: OPB03(1,800T) (3.7 cM) and OPA18(830T) (17.4 cM). OPY20(830C) and OPB03(1,800T), used in association as a codominant pair, allowed the identification of the three genotypic classes with a high degree of confidence. Marker OPY20(830C), which is tightly linked to Co-4, is being used to assist in breeding for resistance to anthracnose.
摘要 利用标记辅助选择将主要抗性基因聚合，可以培育出对炭疽病具有持久抗性的普通菜豆（Phaseolus vulgaris）新栽培品种。为此，有必要鉴定抗性来源和与抗性基因紧密连锁的分子标记。本工作的目的是研究栽培品种 TO（携带 Co-4 基因）对炭疽病的抗性遗传，鉴定与 Co-4 紧密连锁的随机扩增多态性 DNA（RAPD）标记，并将该基因导入栽培品种 Rudá。Rudá x TO 的 F(1)、F(2)、F(2:3)、BC(1)s 和 BC(1)r 群体用炭疽病病原菌 Colletotrichum lindemuthianum 65 号菌株接种。F(2)群体的表型比例（抗性/感病）为 3:1，BC(1)s 为 1:1，BC(1)r 为 1:0，证实 TO 栽培品种对炭疽病的抗性为单基因显性。鉴定出与 Co-4 基因连锁的 6 个 RAPD 标记，其中 4 个位于连锁相：OPY20(830C)（0.0 厘摩[cm]）、OPC08(900C)（9.7 cm）、OPI16(850C)（14.3 cm）和 OPJ01(1,380C)（18.1 cm）；2 个位于排斥相：OPB03(1,800T)（3.7 cm）和 OPA18(830T)（17.4 cm）。OPY20(830C)和 OPB03(1,800T)，作为一对共显性标记，可高度准确地鉴定三种基因型。与 Co-4 紧密连锁的标记 OPY20(830C) 正用于炭疽病抗性的辅助选育。
