Ferguson L R, Parslow M I, McLarin J A
Mutat Res. 1986 Apr-May;170(1-2):47-53. doi: 10.1016/0165-1218(86)90081-9.
The clastogenic potential of the pine tree fungal toxin dothistromin was studied by metaphase chromosome analysis of stimulated human peripheral blood lymphocytes exposed in vitro. The frequency of gaps, breaks, deletions and exchanges was scored in a series of cultures from 3 different donors. 50 cells were analysed for each dose level on coded slides. Testing was performed with and without added metabolic activation (as S9 mix) and aflatoxin B1 was used as a positive control in all experiments. Dothistromin caused a dose-dependent increase in the frequency of gaps and deletions which was not dependent on added metabolic activation. Even at high doses of dothistromin only a very small number of complex exchange-type aberrations were seen. This is in contrast to aflatoxin B1 where such aberrations were seen at low dose levels and especially in cultures to which S9 mix was added. High doses of dothistromin caused culture toxicity manifesting as haemolysis of the donor red blood cells and reduction of mitotic index. Culture toxicity occurred without a marked increase in aberration frequency. This toxicity may be masking any major potential for clastogenicity by dothistromin.
通过对体外暴露于松树真菌毒素多杀霉素的人外周血淋巴细胞进行中期染色体分析，研究了多杀霉素的致断裂潜力。在来自3个不同供体的一系列培养物中，对裂隙、断裂、缺失和交换的频率进行了评分。在编码载玻片上，对每个剂量水平的50个细胞进行分析。在有和没有添加代谢激活剂（作为S9混合物）的情况下进行测试，并且在所有实验中黄曲霉毒素B1用作阳性对照。多杀霉素导致裂隙和缺失频率呈剂量依赖性增加，这与是否添加代谢激活剂无关。即使在高剂量的多杀霉素下，也仅观察到极少数复杂的交换型畸变。这与黄曲霉毒素B1相反，在黄曲霉毒素B1中，在低剂量水平下，尤其是在添加了S9混合物的培养物中，可以观察到这种畸变。高剂量的多杀霉素导致培养物毒性，表现为供体红细胞溶血和有丝分裂指数降低。培养物毒性出现时，畸变频率没有明显增加。这种毒性可能掩盖了多杀霉素任何主要的致断裂潜力。
