Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin, People's Republic of China.
Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, People's Republic of China.
Lipids Health Dis. 2019 Mar 4;18(1):56. doi: 10.1186/s12944-019-0996-5.
ω-3 polyunsaturated fatty acids (PUFAs) are synthesized from α-Linolenic acid (ALA, C18:3ω3) and play important roles in anti-inflammatory and antioxidant responses in mammal cells. ALA is an essential fatty acid which cannot be produced within the human body and must be acquired through diet. The purpose of this study was to evaluate the potential of a novel microalgal strain (HDMA-20) as a source of ω-3 PUFAs including ALA and eicosatetraenoic acid (ETA, C20:4ω3).
Phylogenetic Neighbor-Joining analysis based on 18S ribosomal DNA sequence was used to identify the microalga strain HDMA-20. Autotrophic condition was chosen to cultivate HDMA-20 to reduce the cultivation cost. GC-MS was used to determine the fatty acid composition of HDMA-20 lipid.
A microalgal strain (HDMA-20) from Lake Chengfeng (Daqing, Heilongjiang province, China) was found to accumulate high content of ω-3 PUFAs (63.4% of total lipid), with ALA and eicosatetraenoic acid (ETA, C20:4ω3) accounting for 35.4 and 9.6% of total lipid, respectively. Phylogenetic analysis based on 18S ribosomal DNA sequences suggested that the HDMA-20 belonged to genus Monoraphidium (Selenastraceae, Sphaeropleales) and its 18S rDNA sequence information turned out to be new molecular record of Monoraphidium species. The biomass productivity and lipid content of HDMA-20 were also investigated under autotrophic condition. The biomass productivity of HDMA-20 reached 36.3 mg L day, and the lipid contents was 22.6% of dry weight.
HDMA-20 not only represent an additional source of ALA, but also a totally new source of ETA. The high content of ω-3 PUFAs, especially ALA, of HDMA-20, makes it suitable as a source of nutrition supplements for human health. In addition, HDMA-20 exhibited good properties in growth and lipid accumulation, implying its potential for cost-effective ω-3 PUFAs production in future.
ω-3 多不饱和脂肪酸（PUFAs）是由α-亚麻酸（ALA，C18:3ω3）合成的，在哺乳动物细胞的抗炎和抗氧化反应中发挥重要作用。ALA 是一种必需脂肪酸，人体无法合成，必须通过饮食获得。本研究旨在评估新型微藻菌株（HDMA-20）作为 ω-3 PUFAs（包括 ALA 和二十碳四烯酸（ETA，C20:4ω3）的潜在来源。
基于 18S 核糖体 DNA 序列的系统发育邻接法用于鉴定微藻菌株 HDMA-20。选择自养条件来培养 HDMA-20 以降低培养成本。GC-MS 用于测定 HDMA-20 脂质的脂肪酸组成。
从中国黑龙江省大庆市乘风湖发现一株微藻菌株（HDMA-20），该菌株积累了高含量的 ω-3 PUFAs（总脂质的 63.4%），其中 ALA 和二十碳四烯酸（ETA，C20:4ω3）分别占总脂质的 35.4%和 9.6%。基于 18S 核糖体 DNA 序列的系统发育分析表明，HDMA-20 属于单毛藻属（Selenastraceae，Sphaeropleales），其 18S rDNA 序列信息是单毛藻属物种的新分子记录。还研究了 HDMA-20 在自养条件下的生物量生产力和脂质含量。HDMA-20 的生物量生产力达到 36.3mg/L/天，脂质含量占干重的 22.6%。
HDMA-20 不仅是 ALA 的另一个来源，也是 ETA 的全新来源。HDMA-20 的 ω-3 PUFAs 含量高，特别是 ALA，适合作为人类健康营养补充剂的来源。此外，HDMA-20 在生长和脂质积累方面表现出良好的特性，这意味着它在未来具有生产成本效益高的 ω-3 PUFAs 的潜力。
