Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100039, China.
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Bioresour Technol. 2015;187:221-227. doi: 10.1016/j.biortech.2015.03.125. Epub 2015 Mar 31.
NaCl induction in photoautotrophic two-step cultivation is very promising, but time node and concentration are critical to the entire production. In this study Monoraphidium dybowskii LB50 was subjected to different NaCl concentrations at different growth phases. Results showed that during the initial phase (IP), fixed carbon was used for sugar and lipid under 5gL(-1) NaCl induction, as well as for protein under 10gL(-1) NaCl induction. At late-exponential growth phase (LEGP), the highest lipid productivity was obtained at 20gL(-1) NaCl. At stationary phase (SP) the highest lipid productivity was also under 20gL(-1) NaCl but lower than that of LEGP. In summary, lipid content and quality were improved at all growth phases under NaCl induction. Therefore, cultivation scale can be sued to determine the time node and dosage of the inducer, thereby realizing the economic efficiency of the fundamental guarantee in photoautotrophic two-step cultivation.
NaCl 诱导在光自养两步培养中很有前景，但时间节点和浓度对整个生产至关重要。在这项研究中，对不同生长阶段的单胞藻 LB50 施加了不同浓度的 NaCl。结果表明，在初始阶段（IP），5gL(-1) NaCl 诱导下固定碳用于糖和脂质，10gL(-1) NaCl 诱导下固定碳用于蛋白质。在对数生长后期（LEGP），20gL(-1) NaCl 下获得最高的脂质生产力。在静止期（SP），20gL(-1) NaCl 下也获得了最高的脂质生产力，但低于 LEGP。总的来说，NaCl 诱导下所有生长阶段的脂质含量和质量都得到了提高。因此，可以根据培养规模来确定诱导剂的时间节点和剂量，从而实现光自养两步培养的经济效益的基本保障。
