Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Department of Chemistry and the State Key Laboratory of Molecular Engineering, Fudan University, Shanghai, 200433, People's Republic of China.
Anal Chim Acta. 2019 May 30;1057:145-151. doi: 10.1016/j.aca.2018.12.063. Epub 2019 Jan 14.
Hydrophilic interaction liquid chromatography is a significant strategy for the separation and enrichment of glycoproteins and glycopeptides. A layered imine-based covalent organic polymer with mesopores (denoted as p-TpBDH) was successfully fabricated by a facile solvothermal method. Then p-TpBDH-OH with abundant hydrophilic groups was evolved from p-TpBDH by the direct reduction. 36 and 40 glycopeptides were identified from IgG digests respectively by p-TpBDH and p-TpBDH-OH. Furthermore, the p-TpBDH-OH exhibits superior selectivity (IgG: BSA = 1: 250) for glycopeptides compared with the p-TpBDH. Encouragingly, a total of 463 glycopeptides assigned to 173 glycoproteins were finally identified from only 2 μL human serum by the p-TpBDH-OH. Compared with p-TpBDH, abundant hydrophilic and nitrogen-containing affinity sites of p-TpBDH-OH facilitate effective hydrophilic interaction between the polymeric material and glycopeptides. All the results demonstrate the functionalized hydrophilic covalent organic polymer has great potential in large-scale N-glycoproteomic research.
亲水作用液相色谱法是分离和富集糖蛋白和糖肽的重要策略。通过简便的溶剂热法成功制备了具有介孔的层状亚胺基共价有机聚合物（表示为 p-TpBDH）。然后，通过直接还原将 p-TpBDH 转化为具有丰富亲水性基团的 p-TpBDH-OH。分别通过 p-TpBDH 和 p-TpBDH-OH 从 IgG 消化物中鉴定出 36 和 40 个糖肽。此外，与 p-TpBDH 相比，p-TpBDH-OH 对糖肽表现出更高的选择性（IgG:BSA=1:250）。令人鼓舞的是，通过 p-TpBDH-OH 仅从 2μL 人血清中最终鉴定出 463 个糖肽，这些糖肽分别分配到 173 种糖蛋白中。与 p-TpBDH 相比，p-TpBDH-OH 丰富的亲水性和含氮亲和位点促进了聚合物材料与糖肽之间的有效亲水相互作用。所有结果表明，功能化亲水共价有机聚合物在大规模 N-糖蛋白质组学研究中具有巨大的潜力。
