Cheng Li, Huang Mingxian, Ren Hui, Wang Yiqiang, Cui Hongmei, Xu Mingming
Institute of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 730000, China.
XJTLU Wisdom Lake Academy of Pharmacy-BEAVER Biomedical Joint Laboratory, Suzhou, 215123, China.
Anal Bioanal Chem. 2025 Apr;417(9):1731-1749. doi: 10.1007/s00216-024-05708-9. Epub 2024 Dec 23.
Protein glycosylation is one of the most important post-translational modifications, implicated in the development of various diseases, including neurodegenerative diseases, diabetes, and cancers. However, the low content of glycoproteins in biological samples, the diversity and heterogeneity of glycan structures, and insensitive detection methods make glycosylation analysis challenging. As a result, efficient enrichment of glycopeptides from complex samples is a critical step. Efficient enrichment technology can increase the abundance of intact N-glycopeptides in complex biological samples, thereby improving the sensitivity and coverage of glycosylation analysis, which is of great significance for the accurate identification of biomarkers and the development of glycopeptide-based drugs. Among various separation methods for N-glycopeptides, hydrophilic interaction chromatography has received increasing attention, and a variety of enrichment materials have been developed. This article classifies and describes the relevant hydrophilic interaction chromatography materials and provides a comprehensive review of their applications in N-glycopeptide enrichment regarding selectivity, sensitivity, and enrichment performance. Future development trends of ideal glycopeptide enrichment materials are also discussed.
蛋白质糖基化是最重要的翻译后修饰之一，与包括神经退行性疾病、糖尿病和癌症在内的各种疾病的发生发展有关。然而，生物样品中糖蛋白含量低、聚糖结构的多样性和异质性以及检测方法的不灵敏使得糖基化分析具有挑战性。因此，从复杂样品中高效富集糖肽是关键步骤。高效富集技术可以增加复杂生物样品中完整N-糖肽的丰度，从而提高糖基化分析的灵敏度和覆盖率，这对于生物标志物的准确鉴定和基于糖肽的药物开发具有重要意义。在N-糖肽的各种分离方法中，亲水相互作用色谱受到越来越多的关注，并且已经开发了多种富集材料。本文对相关亲水相互作用色谱材料进行分类和描述，并就其在N-糖肽富集中的选择性、灵敏度和富集性能等应用进行全面综述。还讨论了理想糖肽富集材料的未来发展趋势。
