Agriculture College, Xinyang Agriculture and Forestry University, Xinyang, 464000, P. R. China.
Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences/Integrated Pest Management Center of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding, 071000, P. R. China.
Sci Rep. 2019 Mar 4;9(1):3339. doi: 10.1038/s41598-019-38896-x.
Accurate detection and recognition of chemical signals play extremely important roles for insects in their survival and reproduction. Chemosensory receptors, including odorant receptors (ORs), ionotropic receptors (IRs) and gustatory receptors (GRs), are involved in detection of volatile signals. In the present study, we aimed to identify candidate chemosensory receptors, and RNA-seq technology was employed to sequence the antennal transcriptome of Protaetia brevitarsis (Coleoptera: Scarabaeidae: Cetoniinae), a native agricultural and horticultural pest in East-Asia. According to the sequence similarity analysis, we identified 72 PbreORs, 11 PbreGRs and eight PbreIRs. Among PbreORs, PbreOR2, PbreOR33 and PbreOR53 were preliminarily classified into pheromone receptors. Further qRT-PCR analysis indicated that 11 PbreORs were specifically expressed in the antennae of male P. brevitarsis, whereas 23 PbreORs were specifically expressed in the female antennae. Our results laid a solid foundation for further functional elucidations of insect chemoreceptors, which could be used as the potential targets of pest management.
准确检测和识别化学信号对昆虫的生存和繁殖起着至关重要的作用。化学感觉受体，包括气味受体（ORs）、离子型受体（IRs）和味觉受体（GRs），参与了挥发性信号的检测。本研究旨在鉴定候选化学感觉受体，采用 RNA-seq 技术对东亚本土农业和园艺害虫暗黑鳃金龟（鞘翅目：金龟子科：鳃角金龟亚科）的触角转录组进行测序。根据序列相似性分析，我们鉴定了 72 个 PbreORs、11 个 PbreGRs 和 8 个 PbreIRs。在 PbreORs 中，PbreOR2、PbreOR33 和 PbreOR53 初步被归类为信息素受体。进一步的 qRT-PCR 分析表明，11 个 PbreORs 特异性表达于雄性暗黑鳃金龟的触角，而 23 个 PbreORs 特异性表达于雌性触角。我们的研究结果为昆虫化学感觉受体的进一步功能阐明奠定了基础，这些受体可作为害虫管理的潜在目标。
