Dong Junfeng, Song Yueqin, Li Wenliang, Shi Jie, Wang Zhenying
Forestry College, Henan University of Science and Technology, Luoyang, 471003, China.
Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding, 071000, China.
PLoS One. 2016 Jan 26;11(1):e0147768. doi: 10.1371/journal.pone.0147768. eCollection 2016.
Olfaction plays a crucial role in insect population survival and reproduction. Identification of the genes associated with the olfactory system, without the doubt will promote studying the insect chemical communication system. In this study, RNA-seq technology was used to sequence the antennae transcriptome of Athetis dissimilis, an emerging crop pest in China with limited genomic information, with the purpose of identifying the gene set involved in olfactory recognition. Analysis of the transcriptome of female and male antennae generated 13.74 Gb clean reads in total from which 98,001 unigenes were assembled, and 25,930 unigenes were annotated. Total of 60 olfactory receptors (ORs), 18 gustatory receptors (GRs), and 12 ionotropic receptors (IRs) were identified by Blast and sequence similarity analyzes. One obligated olfactory receptor co-receptor (Orco) and four conserved sex pheromone receptors (PRs) were annotated in 60 ORs. Among the putative GRs, five genes (AdisGR1, 6, 7, 8 and 94) clustered in the sugar receptor family, and two genes (AdisGR3 and 93) involved in CO2 detection were identified. Finally, AdisIR8a.1 and AdisIR8a.2 co-receptors were identified in the group of candidate IRs. Furthermore, expression levels of these chemosensory receptor genes in female and male antennae were analyzed by mapping the Illumina reads.
嗅觉在昆虫种群的生存和繁殖中起着至关重要的作用。毫无疑问，鉴定与嗅觉系统相关的基因将有助于研究昆虫的化学通讯系统。在本研究中，利用RNA测序技术对中国一种新出现的作物害虫异形阿夜蛾的触角转录组进行测序，该害虫的基因组信息有限，目的是鉴定参与嗅觉识别的基因集。对雌、雄触角转录组的分析共产生了13.74 Gb的clean reads，从中组装出98,001个单基因，并对25,930个单基因进行了注释。通过Blast和序列相似性分析，共鉴定出60个嗅觉受体（ORs）、18个味觉受体（GRs）和12个离子型受体（IRs）。在60个ORs中注释了1个必需的嗅觉受体共受体（Orco）和4个保守的性信息素受体（PRs）。在推定的GRs中，5个基因（AdisGR1、6、7、8和94）聚集在糖受体家族中，鉴定出2个参与二氧化碳检测的基因（AdisGR3和93）。最后，在候选IRs组中鉴定出AdisIR8a.1和AdisIR8a.2共受体。此外，通过对Illumina reads进行定位，分析了这些化学感应受体基因在雌、雄触角中的表达水平。
