Rubio-Rincón Francisco J, Welles Laurens, Lopez-Vazquez Carlos M, Abbas Ben, van Loosdrecht Mark C M, Brdjanovic Damir
Sanitary Engineering Chair Group, Department of Environmental Engineering and Water Technology, UNESCO-IHE Institute for Water Education, Delft, Netherlands.
Department of Biotechnology, Delft University of Technology, Delft, Netherlands.
Front Microbiol. 2019 Feb 18;10:125. doi: 10.3389/fmicb.2019.00125. eCollection 2019.
Accumulibacter phosphatis is in general presented as the dominant organism responsible for the biological removal of phosphorus in activated sludge wastewater treatment plants. Lab-scale enhanced biological phosphorus removal (EBPR) studies, usually use acetate as carbon source. However, the complexity of the carbon sources present in wastewater could allow other potential poly-phosphate accumulating organism (PAOs), such as putative fermentative PAOs (e.g., ), to proliferate in coexistence or competition with . Accumulibacter. This research assessed the effects of lactate on microbial selection and process performance of an EBPR lab-scale study. The addition of lactate resulted in the coexistence of . Accumulibacter and in a single EBPR reactor. An increase in anaerobic glycogen consumption from 1.17 to 2.96 C-mol/L and anaerobic PHV formation from 0.44 to 0.87 PHV/PHA C-mol/C-mol corresponded to the increase in the influent lactate concentration. The dominant metabolism shifted from a polyphosphate-accumulating metabolism (PAM) to a glycogen accumulating metabolism (GAM) without EBPR activity. However, despite the GAM, traditional glycogen accumulating organisms (GAOs; Competibacter phosphatis and ) were not detected. Instead, the 16s RNA amplicon analysis showed that the genera was the dominant organism, while a quantification based on FISH-biovolume indicated that . Accumulibacter remained the dominant organism, indicating certain discrepancies between these microbial analytical methods. Despite the discrepancies between these microbial analytical methods, neither . Accumulibacter nor performed biological phosphorus removal by utilizing lactate as carbon source.
聚磷菌通常被认为是活性污泥废水处理厂中负责生物除磷的主要微生物。实验室规模的强化生物除磷（EBPR）研究通常使用乙酸盐作为碳源。然而，废水中存在的碳源的复杂性可能会使其他潜在的聚磷微生物（PAOs），如假定的发酵型PAOs（如 ），与聚磷菌共存或竞争并增殖。本研究评估了乳酸对EBPR实验室规模研究中微生物选择和工艺性能的影响。添加乳酸导致聚磷菌和 在单个EBPR反应器中共存。厌氧糖原消耗量从1.17 C-mol/L增加到2.96 C-mol/L，厌氧聚-β-羟基戊酸（PHV）的形成从0.44 PHV/PHA C-mol/C-mol增加到0.87 PHV/PHA C-mol/C-mol，这与进水乳酸浓度的增加相对应。在没有EBPR活性的情况下，主要代谢从聚磷代谢（PAM）转变为糖原积累代谢（GAM）。然而，尽管存在GAM，但未检测到传统的糖原积累微生物（GAOs；磷酸盐竞争菌和 ）。相反，16s RNA扩增子分析表明 属是主要微生物，而基于荧光原位杂交-生物体积的定量分析表明聚磷菌仍然是主要微生物，这表明这些微生物分析方法之间存在一定差异。尽管这些微生物分析方法之间存在差异，但聚磷菌和 均未利用乳酸作为碳源进行生物除磷。
